Experimental Cell Biology 2

Provides a summary on basic laboratory techniques, such as: DNA isolation, DNA manipulation and molecular cloning, PCR, RT-PCR (qPCR), NASBA, Site-directed mutagenesis, Sanger sequencing, Chromatography, SDS-PAGE, Fluorescent labels/probes, Co-immunoprecipitation, knock-out mice, RNA interference an...

This document provides a summary of the following subjects: Microbiome, microbiota, phylogeny, taxonomy, microbes, biofilms, gastrointestinal microbiota, gut microbiota, oral microbiota, Porphyromonas gingivalis, urogenital microbiota, skin microbiota, antibiotics, faecal bacteriotherapy, Koch’s p...

This document provides a summary of the following subjects: intracellular pathogens, bacteria, human kinome, Salmonella thyphimurium, structure-activity relationship (SAR), molecular mechanisms of Salmonella, small GTPases, intracellular transport in Salmonella, Phosphoinositides, endosomes, AKT, Ra...

This document contains an overview of the discovery and pathway of apoptosis. In this summary literature articles are used and linked.

This document provides a summary of the lecture Single-cell gene expression. Topics that are discussed are: mRNA, mRNA expression, Northern blot, qPCR, RNA sequencing, DNA microarray, gene expression, single-cell gene expression analysis, High-throughput single-cell RNA analysis methods, Low through...

This document provides a summary of proteomic techniques, such as mass spectrometry. The focus lies on tandem MS, peptide fragmentation, MS/MS, Maldi and electrospray, Time-of-flight, orbitrap and the pathology of Alzheimer’s disease and how the MS contributes to the research. There is also a brie...

This document provides a summary of the whole Experimental Cell Biology 2 course. **You can also find the separate lectures on my profile! Topics that are discussed are: Endothelial cells (leukocyte extravasation, chemokines, and stem cell homing), Basic techniques in cell biology (PCR, chromat...