Molecular Diagnostics Bundle Set

3 common parts of a nucleotide - Answer 1) 5 carbon sugar 2) phosphate group 3) nitrogenous base Purines - Answer Adenine & Guanine Pyrimdines - Answer Cytosine, Uracil, and Thymine Nucleotide subunits are linked together by - Answer Phosphodiester bonds List three structural...

in one nucleotide of DNA what are the 3 major moieties (components) that make it up? - Answer - 5 carbon sugar - phosphate group - nitrogen bases what is the overall charge of DNA and on which moiety is it found - Answer DNA is overall negative, it is found on the oxygen of phosphate backbo...

What are the two major classes of nucleic acids? - Answer 1. deoxyribonucleic acid (DNA) 2. Ribonucleic acid (RNA) What is structure of DNA? - Answer phosphodiester bonds connect deoxyribose sugars What are the bases in DNA? - Answer adenine guanine cytosine thymine What are the ...

DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent bond? - Answer Phosphodiester Nucleotides in DNA consist of what? - Answer Nitrogen Base, deoxyribose, and phosphate Which of the five ribose carbons of the deoxyribonucleotide are involved in formation...

trait - Answer particular characteristic Which elements make up the macromolecule of DNA? - Answer carbon, nitrogen, oxygen, phosphorous, and hydrogen What is the structure of DNA? - Answer phosphorylated ribose sugar (deoxy ribose) backbone with a nitrogenous base (guanine, adenine, cyt...

A micoarray is testing for: - Answer a lot of genetic characteristics from one sample Southern Hybridization - Answer extract DNA probe w/ DNA Northern Hybridization - Answer extract RNA probe w/ DNA Western Hybridization - Answer extract proteins probe with antibodies What...

DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent bond? A. aldehyde B. ester C. phosphodiester D. peptide - Answer phosphodiester What do phosphoiester bonds attach? - Answer hydroxyl oxygen of one phosphorylated ribose sugar to the phosphate phosphor...

Molecular Diagnostics - Answer Combination of laboratory medicine, molecular genetics and technology Molecular Diagnostics in Health Care - Answer Techniques allow detection of pathogenic mutations in nucleic acids to facilitate detection, diagnosis, classification, prognosis and therapy moni...

List the different specimens that may be submitted to a molecular diagnostics lab for testing - Answer Blood, Urine, stool, amniotic fluid, CSF, buccal cells. List 2 common anticoagulants used for molecular testing - Answer EDTA (for HIV, HCV, and CMV testing) and Acid Citrate Dextrose (for c...

Starting material, ending material, and active enzyme in process of replication is: - Answer DNA, DNA, DNA Polymerase Approximately how many genes are present in the human genome: - Answer 20,000-35,000 What type of sugar molecule is present in DNA - Answer Deoxyribose What are the fo...

Which sample types must be amplified prior to analysis? - Answer buccal cells, saliva, bone, teeth, hair, dried blood, fixed tissue What are two ways to break down cell walls for DNA extraction? - Answer 1. homogenization through physical grinding 2. treatment of detergent (SDS) with strong ...

d (primers) - Answer *The specificity of the PCR reaction is determined by which of the following components?* a. Mg2+ concentration b. Deoxynucleotides c. DNA polymerase d. Primers a (primer dimers) - Answer *Complementarity of primer pairs at the 3' ends will result in what artifacts...

The advantages of using real-time PCR to detect Chlyamidia and Gonorrhea is... - Answer - allow detection of organisms that are hard to culture - can be automated for high volume processing MALDI-TOF improves traditional culture methods by... - Answer -reduces need for biochem tests -pathog...

The analysis of external specimens to assess technologist competency and assay performance (CAP survey) is which kind of testing? - Answer Proficiency A woman calls and asks that the results of her husband's HIV viral load assay be faxed to her. She gives the technologist her husband's name a...