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MLS Review: Immunohematology| 135 QUESTIONS WITH COMPLETE SOLUTIONS
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Phenotyping Correct Answer: the physical expression of a genotype Dosage Correct Answer: an antibody reacting stronger with homozygous cells than with heterozygous cells Antigens that exhibit dosage Correct Answer: Kell, M, N, S, s, Fya, Fyb, Jka, Jkb, antigens of the Rh system Fy(a-b-)...

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MLS Review: Immunohematology| 135 QUESTIONS
WITH COMPLETE SOLUTIONS
Phenotyping Correct Answer: the physical expression of a genotype
Dosage Correct Answer: an antibody reacting stronger with homozygous cells than with heterozygous cells
Antigens that exhibit dosage Correct Answer: Kell, M, N, S, s, Fya, Fyb, Jka, Jkb, antigens of the Rh system
Fy(a-b-) phenotype Correct Answer: occurs in almost 70% of African americans and is very rare
in whites
Xga antigen Correct Answer: X-linked and expressed more frequently in women than in men
Genotype that will give rise to the Bombay phenotype Correct Answer: hh
Meiosis in cell division is limited to the ova and sperm producing four gametes containing this complement of DNA. Correct Answer: 1N
Interphase Correct Answer: the stage in between cell divisions
Describes the expression of most blood group antigens Correct Answer: Codominant
indirect/secondary/second order exclusion Correct Answer: occurs when a genetic marker is absent in the child but should have been transmitted by the alleged father
Linkage disequilibrium Correct Answer: a phenomenon in which alleles situated in close proximity on a chromosome associate with one another more than would be expected from individual allelic frequencies
In the Hardy-Weinberg formula, p^2 represents Correct Answer: the homozygous population of one allele
Hardy-Weinberg formula Correct Answer: p^2 + 2pq + q^2
In this type of inheritance, the father carries the trait on his X chromosome. All his daughters will express the trait. Correct Answer: X-linked dominant
IgM antibodies have the ability to directly agglutinate RBCs and cause visible agglutination because Correct Answer: IgM antibodies are larger molecules and have the ability to bind more antigen. Enhancement medium that decreases the zeta potential, allowing antibody and antigen to come closer together Correct Answer: Low ionic strength solution (LISS)
Analogous to an anamnestic antibody reaction Correct Answer: Secondary immune response; memory lymphocytes respond rapidly to foreign antigen in producing specific IgG antibodies
Antibodies to a component of complement contained in the rabbit polyspecific anti-human globulin (AHG) reagent for detection of in vivo sensitization. Correct Answer: Anti-IgG and anti-C3d
The only ABO phenotype incompatible with O cells Correct Answer: Bombay
Antibodies formed by a Bombay phenotype individual Correct Answer: Anti-A, B, and H
The acquired B phenomenon is only seen in Correct Answer: Group A persons
Blood group that has the least amount of H antigen Correct Answer: A1B
Type of RBCs that can be transfused to an A2 person with anti-A1 Correct Answer: A or O
Should be done if all forward and reverse ABO results as well as AC are positive Correct Answer: wash the cells with warm saline, and autoadsorb the serum at 4C
Should be done if all forward and reverse ABO results are negative Correct Answer: incubate at 22C or 4C to enhance weak expression
N-acetyl-D-galactosamine is the immunodominant carbohydrate that reacts with Correct Answer: Dolichos biflorus
Reaction that would be the same for an A1 and A2 person Correct Answer: Positive reaction with Anti-A,B
Rh positive refers to Correct Answer: the presence of D antigen
Donor unit selected for recipient with anti-c Correct Answer: r'r^y
r' Correct Answer: dCe
r^y Correct Answer: dCE
Genotype that usually shows the strongest reaction with anti-D Correct Answer: D--/D--
Testing for Rh antigens and antibodies vs ABO testing Correct Answer: ABO reactions are primarily caused by IgM antibodies and usually occur at room temperature; Rh antibodies are IgG and agglutination usually requires a 37C incubation and enhancement media

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