Biochemistry Lab Report
BIOCHEMISTRY LAB REPORT 4 2023/2024| PORTAGE
LEARNING|WITH PROCEDURES, EXPLANATION AND
FINAL RESULTS.HIGHLY VERIFIRD
Experiment #: Lab 4
Title: Chromatography of molecules.
Purpose: The goals for the student performing the experiment are 1. Explain the
fundamentals of chromatography. 2. Demonstrate ion exchange and size-
exclusion chromatography. 3. Separate a Real-World sample. The student should
be able to use the techniques learned and be able to apply them to further use in
the lab or in a chemistry class again. Students should be able to fully recall and
demonstrate how to do these techniques when completed the lab.
Procedure:
Part 1:
• Setting up the columns.
o Notes: The pH of a solution can impact the stationary phase and the
molecules to be separated.
o 1. The chromatographic media is poured in as a slurry.
o 2. The media is permitted to settle.
o 3. Buffer (mobile phase) is permitted to flow through the column.
• Procedure:
o Step 1: Put on appropriate lab equipment based on what solutions
and chemicals will be used in the lab that they are performing. Ex:
Gloves, Lab coat, Closed toe shoes, Protective glasses, etc. Also
have the appropriate materials needed for completion of the lab.
o Step 2: Place glass wool in column so that the media has a barrier to
keep it from eluting.
o Step 3: Place column on stand. Be sure to label columns.
o Step 4: With prepared media place in column and let it settle for a
couple of minutes.
o Step 5: Let some of the buffer for the columns run through so that
they are in the gel. Never let the gel go dry, always keep the buffer
above the gel.
o Step 6: Your columns are not set up for separating test mixture
compounds.
Part 2:
• Separation of two test mixtures.
o Notes:
▪ There will be two test mixtures applied to each column.
o Step 1: Put on appropriate lab equipment based on what solutions
and chemicals will be used in the lab that they are performing. Ex:
Gloves, Lab coat, Closed toe shoes, Protective glasses, etc. Also
have the appropriate materials needed for completion of the lab.
o Step 2: With columns already set up and have had buffer ran through
, Biochemistry Lab Report
them know you are ready to begin separating test mixtures.
BIOCHEMISTRY LAB REPORT 4 2023/2024| PORTAGE
LEARNING|WITH PROCEDURES, EXPLANATION AND
FINAL RESULTS.HIGHLY VERIFIRD
Experiment #: Lab 4
Title: Chromatography of molecules.
Purpose: The goals for the student performing the experiment are 1. Explain the
fundamentals of chromatography. 2. Demonstrate ion exchange and size-
exclusion chromatography. 3. Separate a Real-World sample. The student should
be able to use the techniques learned and be able to apply them to further use in
the lab or in a chemistry class again. Students should be able to fully recall and
demonstrate how to do these techniques when completed the lab.
Procedure:
Part 1:
• Setting up the columns.
o Notes: The pH of a solution can impact the stationary phase and the
molecules to be separated.
o 1. The chromatographic media is poured in as a slurry.
o 2. The media is permitted to settle.
o 3. Buffer (mobile phase) is permitted to flow through the column.
• Procedure:
o Step 1: Put on appropriate lab equipment based on what solutions
and chemicals will be used in the lab that they are performing. Ex:
Gloves, Lab coat, Closed toe shoes, Protective glasses, etc. Also
have the appropriate materials needed for completion of the lab.
o Step 2: Place glass wool in column so that the media has a barrier to
keep it from eluting.
o Step 3: Place column on stand. Be sure to label columns.
o Step 4: With prepared media place in column and let it settle for a
couple of minutes.
o Step 5: Let some of the buffer for the columns run through so that
they are in the gel. Never let the gel go dry, always keep the buffer
above the gel.
o Step 6: Your columns are not set up for separating test mixture
compounds.
Part 2:
• Separation of two test mixtures.
o Notes:
▪ There will be two test mixtures applied to each column.
o Step 1: Put on appropriate lab equipment based on what solutions
and chemicals will be used in the lab that they are performing. Ex:
Gloves, Lab coat, Closed toe shoes, Protective glasses, etc. Also
have the appropriate materials needed for completion of the lab.
o Step 2: With columns already set up and have had buffer ran through
, Biochemistry Lab Report
them know you are ready to begin separating test mixtures.
