CMMB 343 lab final exam questions and answers with complete solution

CMMB 343 lab final exam questions and answers with complete solution How to do ..... BHI stant How to interpret results? - Answer ️️ -Slanted BHI agar in a test tube Inoculate with the sample Will see growth on surface of agar if aerobic microbe. Fissures and cracks below surface if able to ferment. Deep shake culture - Answer ️️ -Innoculate a loop of sample in molten agar and rotate in hands to mix. If fissures below the surface, can ferment. If no fissures, cannot ferment. Thioglycollate Medium - Answer ️️ -Pipette some liquid culture to the bottom of the tube BELOW the pink layer. Turbidity will show where the microbes are able to survive. This is based on the oxygen gradient within the tube. A facultative aerobe will show what results in a thioglycollate tube? - Answer ️️ -Turbidity all throughout the tube, but a bit more turbid in the top segment. What is the upper pink layer of a thioglycollate tube? - Answer ️️ -oxygenated layer When they say "oxygen is a nutrient" what is meant? - Answer ️️ -It is needed as the final e- acceptor in the ETC to generate ATP. Name 2 enzymes that are part of respiration. What 2 tests can be used to detect them? - Answer ️️ -cytochrome c oxidase Oxidase test catalase Catalase test How to do and interpret.... oxidase test - Answer ️️ --hold the RED end of oxidase stick -put BROWN end into cells Negative result: colourless Positive result: blue How to do and interpret.... catalase test - Answer ️️ -Add 2 drops of hydrogen peroxide to a colony on an agar plate if bubbling, catalase is present What are the gas bubbles you see in a positive catalase test? - Answer ️️ -O2 The enzyme catalase converts the added Hydrogen Peroxide to ___________ and ___________. - Answer ️️ -water and oxygen gas How to do and interpret.... H&L glucose test - Answer ️️ -use 2 H&L glucose test tubes. These have glucose as a carbon source in the medium. Shows if and where the species can use glucose as a c