AQA A-Level biology- Recombinant DNA Technology Midterm Exam Solved Correctly 2024.

What does recombinant DNA technology involve? - Answer Transfer of fragments of DNA from one organisms or species to another What can recombinant DNA technology be used for? - Answer Medicine: producing large amounts of a gene product e.g. protein Agriculture: genetically engineer crops Why can DNA be transferred between organisms? - Answer Genetic code is universal and so are the transcription and translation factors Give three examples of how fragments of DNA can be produced - Answer Using restriction enzymes to cut out gene from DNA Conversion of mRNA to complementary DNA Using a gene machine Describe how a restriction enzyme is used to obtain a DNA fragment - Answer Cut the fragment from the donor DNA using a restriction endonuclease The enzyme cuts the DNA at a specific palindromic sequence of bases Describe how cDNA is produced - Answer Extract mRNA from a cell where the gene is expressed