Biochem Engineering: Bioseparations Exam Study Guide.

Biochem Engineering: Bioseparations Exam Study Guide. Depth filtration - answerdifferent layers of filtration as it goes deeper, particles retained within its structure not on surface surface filtration - answerfixed pore size that stops larger microorganisms on the surface continuous centrifugation advantages - answer1. high efficiency in separation from supernatant 2. continuous 3. no clogging continuous centrifugation disadvantages - answer1. high maintenance 2. aerosol formation 3. high cost products that need cell disruption - answer1. lipids and carbs for vaccines like meningitis 2. nucleic acids (RNA therapeutics) 3. virus like particles (HepB) 4. recombinant proteins (interferons) high pressure homogenizer - answerpressure change and high shear through orifice breaks cells; very effective but can result in high temps high speed ball mix - answerblender with steel balls that takes a long time and a lot of energy enzymatic lysis - answer1. degrading enzymes 2. detergents solubilize lipid of membrane 3. for industrial chemical production 4. concerns about remains for therpuetics ultrasound - answer1. treatment of cells in