Micro Bio Lab Final TWU Premium Quality Solutions, Assured Top Academic Performance, Verified and Proven

Direct methods - Answer count viable cells that reproduce to form colonies colony forming units per milliliter - Answer because a colony may arise from more than one colony, especially with cells that form clusters, pairs or chains, organisms are quantified using _________ indirect methods - Answer counting cells which include turbidity of a liquid sample with a spectrometer, metabolic activity, and dry weight Dilution factor - Answer the ratio of the initial and final solution volumes pour plate method - Answer A method of inoculating a solid nutrient medium by mixing bacteria in the melted medium and pouring the medium into a Petri dish to solidify spread plate method - Answer a plate count method in which inoculum is spread over the surface of a solid culture medium Quebec colony counter - Answer a light box with a magnifying lens to closely observe and count the colonies CFU - Answer colony forming units (estimated number of variable bacteria or fungal cells in a sample) cidal agents - Answer agents whcih kill cells static agents - Answer agents which inhibit the growth of cells with out killing them sterilization - Answer referes to the complete destruction/ elimination of all viable organisms in or on a substance being sterilized heat - Answer most important, easily and widely used method to achieve sterilization Incineration - Answer this is using fire, to burn items and physically destroy microbes boiling - Answer 100 C for 30 min will disinfect water. kills vegetative cells but not endospores sterilize - Answer to make free from microorganisms autoclave - Answer most effective and most efficient means of sterilization. 121 C 15 psi 30 min thermal death time - Answer shortest length of time required to kill all test microbes at a specified temperature dry heat - Answer kills cells by destructive oxidation. time and temp no moisture 2 | P a g e low temperature - Answer most organisms grow very little or not at all or below freezing temps or at 0C gamma rays - Answer ionizing- Electromagnetic waves with the shortest wavelengths and highest frequencies uv rays - Answer nonionizing radiation; does not go through things, affects DNA antiseptics - Answer microbicidal agents that are applied to the skin and mucous membranes and should not be taken internally such as ethanol or iodine solutions disinfectants - Answer antimicrobial agents that have varying degrees of cidal activity on microbes and are used on inanimate surfaces such as a tables or floors antimicrobial drugs - Answer a drug used to treat an infectious agent in a person by killing or inhibiting microbial growth chemotherapeutic agents - Answer -chemical agents used to treat disease -destroy pathogenic microbes or inhibit their growth within host -most are antibiotics antibiosis - Answer active inhibitory compound antibiotic - Answer a substance produced by microorganisms that in small amounts inhibits another microorganism broad spectrum - Answer antibiotics which are effective against a wide range of both gram positive and gram negative bacteria narrow specrum - Answer antibiotics which are effective against only a restricted number of bacteria mode of action - Answer the.....