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LECTURE NOTES: VIDEO PART 1 OF BIOCHEMISTRY (BIOSCI98) AT UCI $0.00
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LECTURE NOTES: VIDEO PART 1 OF BIOCHEMISTRY (BIOSCI98) AT UCI
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Notes on lecture video part 1 of Biochemistry course (BIOSCI98) at University of California, Irvine. Completed between weeks 4 and 6 of Winter 2018 quarter.

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  • August 19, 2024
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  • 2018/2019
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Part 1 - Video Lecture Notes
Tuesday, February 5, 2019 4:42 PM




Column chromatography


• Fraction: a set of volume collected after every purification step.


○ Proteins that interact more with the stationary phase will be present in the later fractions.


○ Proteins that interact less with the stationary phase will be present in the earlier fractions.


• @ different pHs, proteins will have different charges.


○ pH > pI: proteins are (-) charged. Low [H] -> proteins will deprotonate into environment.


○ pH < pI: proteins are (+) charged. High [H] -> proteins will be protonated by environment.


• To determine which proteins are in which fractions, you would measure the activity of each fraction.




Protein that will elute in fractions…



Column Early Middle Late


Gel filtration Large Intermediate Small


Cation exchanger Anions Neutral ions Cations


Anion exchanger Cations Neutral ions Anions


Affinity Column Low affinity Not affected by ligands High affinity

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