MCB 2610 Exam 2 Questions and Answers UPDATED 2024 Graded A
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Course
MCB 2610
Institution
MCB 2610
FISH - Cells fixed in place
Fluorescently labeled probes consisting of 165 rRNA gene sequence sent to attach to RNA
View in Epifluorescence microscope
Cultivation independent methods - DNA from unculturable bacteria can be amplified and sequenced by PCR
Sequences can be used to produce fluore...
MCB 2610 Exam 2 Questions and
Answers UPDATED 2024 Graded A
POSSIBLEA
MCB
.
, MCB 2610 Exam 2
FISH - Cells fixed in place
Fluorescently labeled probes consisting of 165 rRNA gene sequence sent to attach to
RNA
View in Epifluorescence microscope
Cultivation independent methods - DNA from unculturable bacteria can be amplified and
sequenced by PCR
Sequences can be used to produce fluorescent probes that will bind to complementary
DNA (Fluorescent In Situ Hybridization FISH)
Microbial consortia - Microbes that can't grow on their own
Metagenomics - DNA is isolated from an environmental sample and sequenced
Metagenomic information must still be confirmed in cultured organisms
Direct counts - Load known volume into gridded slide
Count under a light microscope
Flow Cytometry - Microbial suspension forced through small orifice with a laser beam
Movement of microbe through orifice impacts electric current that flows through orifice
Instances of disruption of current are counted
Specific antibodies can be used to determine size and internal complexity
Spread plate technique - used to get a living (viable) cell count
Small amount of sample is pipetted onto a solid medium then spread around that
medium
Measured in CFU colony forming units
Pour Plate - Original sample is diluted multiple times and the most dilute ones are
poured into a plate with liquid agar
Isolated cells grow into colonies on the surface and inside of the medium
Measured in CFU colony forming units
Calculating CFU/mL - Average cell number / (dilution X volume plated)
What if cells are already very diluted? - A filter apparatus can concentrate cells
Sample is filtered through a membrane filter then the contents that don't go through are
transferred to a medium and incubated
Turbidity - Spetrophotometer sends light through a liquid culture
MCB 2610 Exam 2
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