Lab 1 Introduction to Science
v v v v BIO250L
Student vName: vHaley vLeach
Access vCode v(located von vthe vunderside vof vthe vlid vof vyour vlab vkit): vAC-HMZEAY5
Pre-Lab vQuestions
1. What vconstitutes vpersonal vprotective vequipment? vWhen vshould vpersonal vprotective
v equipment vbe vworn? vWhy vis vpersonal vprotective vequipment vimportant?
PPE vprotects vyour vskin vand vother vsensitive vareas vlike vyour vnose, veyes, vand vmouth. v It vis va
v barrier vbetween vyou vand vprotentailly vharmful vmircrorganisms, vcaustic vchemicals, vand
v biohazards. v In vthis vlab vI vwill vbe vusing vsafety vgoggles, vgloves, vand va vlab vcoat. v PPE vshould vbe
v worn vat vall vtimes vin vthe vlab.
2. Why vare vthere vincreasing vlevels vof vbiological vcontainment vprocedures? vWhat vlevel vof
v biological vcontainment vwill vbe vnecessary vto vcomplete vthe vexperiments vin vthis vmanual?
The vincreasing vlevels vof vbiological vcontainment vare vwarn vand veducate vyou von vthe vdegree vof
v danger vthat va vbiological vpathogen vmay vpose vwhen vyou vare vhanding vit vin vthe vlab. v Every vlevel
v gives va vdescription, va vsample vorganism, va vpathogen vtype, vand vautoclave vrequirements. v Only
v BSL-1 vprocedures vwill vbe vused vin vthis vlab.
3. What vdocument vshould vyou vrefer vto vif vyou vhave vquestions vregarding vthe vsafety vor vdisposal
v of va vchemical? vWhere vcan vthis vdocument vbe vlocated?
The vmaterial vdata vsafety vsheet vis vfound von vthe veScience vLabs vwebsite.
4. List vfive vsafety vprocedures vthat vshould valways vbe vadhered vto vwhen vworking vwith
vmicroorganisms.
a. No vsnacking vor vchewing vgum.
b. PPE vmust vbe vworn vat vall vtime. v(i.e. vapron, vgloves, vmask)
c. Close vtoed vshoes.
d. Long vhair vshould vbe vput vup
e. Clean vlab vtable/surface vwith v10% vplase vor visopropyl valcohol vbefore vand vafter
vexperiments
, Lab 1 Introduction to Science
v v v v BIO250L
v v v v BIO250L
Student vName: vHaley vLeach
Access vCode v(located von vthe vunderside vof vthe vlid vof vyour vlab vkit): vAC-HMZEAY5
Pre-Lab vQuestions
1. What vconstitutes vpersonal vprotective vequipment? vWhen vshould vpersonal vprotective
v equipment vbe vworn? vWhy vis vpersonal vprotective vequipment vimportant?
PPE vprotects vyour vskin vand vother vsensitive vareas vlike vyour vnose, veyes, vand vmouth. v It vis va
v barrier vbetween vyou vand vprotentailly vharmful vmircrorganisms, vcaustic vchemicals, vand
v biohazards. v In vthis vlab vI vwill vbe vusing vsafety vgoggles, vgloves, vand va vlab vcoat. v PPE vshould vbe
v worn vat vall vtimes vin vthe vlab.
2. Why vare vthere vincreasing vlevels vof vbiological vcontainment vprocedures? vWhat vlevel vof
v biological vcontainment vwill vbe vnecessary vto vcomplete vthe vexperiments vin vthis vmanual?
The vincreasing vlevels vof vbiological vcontainment vare vwarn vand veducate vyou von vthe vdegree vof
v danger vthat va vbiological vpathogen vmay vpose vwhen vyou vare vhanding vit vin vthe vlab. v Every vlevel
v gives va vdescription, va vsample vorganism, va vpathogen vtype, vand vautoclave vrequirements. v Only
v BSL-1 vprocedures vwill vbe vused vin vthis vlab.
3. What vdocument vshould vyou vrefer vto vif vyou vhave vquestions vregarding vthe vsafety vor vdisposal
v of va vchemical? vWhere vcan vthis vdocument vbe vlocated?
The vmaterial vdata vsafety vsheet vis vfound von vthe veScience vLabs vwebsite.
4. List vfive vsafety vprocedures vthat vshould valways vbe vadhered vto vwhen vworking vwith
vmicroorganisms.
a. No vsnacking vor vchewing vgum.
b. PPE vmust vbe vworn vat vall vtime. v(i.e. vapron, vgloves, vmask)
c. Close vtoed vshoes.
d. Long vhair vshould vbe vput vup
e. Clean vlab vtable/surface vwith v10% vplase vor visopropyl valcohol vbefore vand vafter
vexperiments
, Lab 1 Introduction to Science
v v v v BIO250L
