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ASU BIO 340 Exam 3 Questions With Answers
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ASU BIO 340 Exam 3 Questions With Answers
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ASU BIO 340 Exam 3 Questions WithAnswers
Describe cthe cexperiments cthat cled cto cthe cdiscovery cof cDNA cas cthe chereditary
cmeterial c- ccorrect canswers✔✔1. cThe cbacterial ctransforming cprinciple cby cGriffith:
found cthat cnonvirulent cR-strain ctakes cup ca ctransforming cprinciple cfrom cthe cvirulent
cheat-killed cS-strain. cThis callowed cthe cR-strain cto ctransform cinto cS-strain cbecoming
cvirulent.
2. cAvery-MacLeod-McCarty cExperiment:
S-strain cfiltrate cwas cmade cand ctreated cwith cRNase, cDnase, cProtease, cand
cCarboase. cThose cwere cthen ctreated cwith cthe cR-strain cand cfound cthat cthe conly
cdample ccontaining cboth cboth cstrains cwas cthe cone cwith cDnase.
3. cThe cHershey cChase cexperiment:
Officially cconcluded cthat cDNA cwas cgenetic cmaterial cfrom cwork cwith cphages.
What cwere cthe cconclusions cfor cthe cthree cexperiments cthat cled cto cDNA cbeing cthe
chereditary cmeterial? c- ccorrect canswers✔✔1. cThe cbacterial ctransforming cprinciple
cby cGriffith:
found cthat cnonvirulent cR-strain ctakes cup ca ctransforming cprinciple cfrom cthe cvirulent
cheat-killed cS-strain. cThis callowed cthe cR-strain cto ctransform cinto cS-strain cbecoming
cvirulent.
2. cAvery-MacLeod-McCarty cExperiment:
DNA cis cthe ctransforming cfactor
3. cThe cHershey-Chase cExperiment:
DNA cis cthe cgenetic cmaterial
What care cthe cbuilding cblocks cof cDNA c- ccorrect canswers✔✔nucleotides
What care cnucleotides ccomposed cof? c- ccorrect canswers✔✔5 ccarbon csugar,
cphosphate cgroup, cnitrogenous cbase
A, cT, cG, cC
Nucleotides ccombine cvia___ c- ccorrect canswers✔✔covalent cbonds
Nucleotides care cassembled cinto cpolynucleotide cchains cby:
a. cendonuclease
b. cDNA cpol. c
c. cRNA cpol.
d. chelicase c- ccorrect canswers✔✔b. cDNA cpol
Who cdetermined cthat cstructure cof cDNA? c- ccorrect canswers✔✔Crick cand cWatson
, Who cdiscovered cthe csecondary cstructure cof cproteins cusing cx-ray ccrystallography? c-
ccorrect canswers✔✔Linus cPauling
Does cDNA crun cparallel cor canti cparallel? c- ccorrect canswers✔✔anti-parallel c(strand
cone c5'-3')(strand c2 c3'-5')
What cis cChargaff's crule? c- ccorrect canswers✔✔DNA cshould chave ca c1:1 cratio(G=C,
cA=T)
The chaploid chuman cgenome cis c3 cGbp clong c(3x10^9 cbp). cThere care capproximately
c50 ctrillion ccells cin cthe chuman cbody. cHow clong cwould call cof cthe chuman cgenomic
cDNA cin cyour cbody cbe cif cit cwere cstretch cout cend cto cend? c- ccorrect
canswers✔✔~2m
There cis capprox, c50 ctrillion ccells cin cthe chuman cbody. cHow clong cwould call cof cthe
chuman cDNA cin cyour cbody cbe cif cit cwere cstretched cend cto cend?
a. c10 cbillion ckm
b. c100 cbillion ckm
c. c1000 cbillion ckm c- ccorrect canswers✔✔b. c100 cbillion ckm
what cis cthe cdifference cin cthe corigins cof creplication cbetween cpro/eukaryotes? c-
ccorrect canswers✔✔prokayotes chave cjust cone cOoR cwhile cEukaryotes chave cmultiple
cdue cto ctheir cbeing cmore ccomplex cthan cprokaryotes.
what cis cthe cexpansion caround cthe corigin cor creplication ccalled? c- ccorrect
canswers✔✔replication cbubble
What cdoes cDNA cpolymerase cdo? c- ccorrect canswers✔✔adds cnucleotides cto cthe
cgrowing cDNA cchain cthat care ccomplementary cto cthe ctemplate cstrand
In cprokaryotes cthere care cthree cDNA cpol. cDescribe cwhat ceach cone cdoes c- ccorrect
canswers✔✔DNA cpol cI cand cII: crepairs
DNA cpol cIII: cDNA csynthesis
How cdoes creplication cknow cwhere cto cbeing? c- ccorrect canswers✔✔There care
cspecific cnucleotide csequences cthat ccode cfor cwhere creplication cbegins.
What cdoes cHelicase cdo cand cwhat cdoes cit crequire? c- ccorrect
canswers✔✔//Helicase// c*unwinds* cthe cDNA cby cbreaking cthe cH-bonds cbetween
cbase cpairs.
//Required// cis cATP chydrolysis
As cthe cDNA cis cunwound cby c*Helicase*, ca cy-shaped cstructure ccalled c_____1____
care cformed.
two c_____2_____ care cformed cat cthe corigin cor creplication cand cthese cget cextended
c____3____ c- ccorrect canswers✔✔1 cand c2 creplication cforks
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