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TCU Microbiology Lab Exam 1 Questions and Answers 100% Pass
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TCU Microbiology Lab Exam 1 Questions and Answers 100% Pass chemically defined (synthetic) media - media composed of precisely defined chemicals elements that all bacteria need - carbon, nitrogen, phosphorus, sulfur, oxygen, and hydrogen minimal media - media that contains exact nutrients nee...

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  • January 17, 2025
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  • 2024/2025
  • Exam (elaborations)
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  • tcu microbiology lab exam 1 questions and answers

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TCU Microbiology Lab Exam 1
Questions and Answers 100% Pass


chemically defined (synthetic) media - ✔✔media composed of precisely defined

chemicals


elements that all bacteria need - ✔✔carbon, nitrogen, phosphorus, sulfur, oxygen, and

hydrogen


minimal media - ✔✔media that contains exact nutrients needed for growth and nothing

extra


- media is useful b/c we know exactly what's in it, but few organisms are resourceful

enough to survive with only a few salts and sugars


complex media - ✔✔- it contains all the nutrients necessary for the microbes


- aka general purpose media, satisfies the nutritional requirements of bacteria with

more stringent growth requirements


- consists of mix of digests & extracts from plant or animal tissue


- nutrient rich but poorly defined with the exact nutrients unknown




Katelyn Whitman, All Rights Reserved © 2025 1

,- in lab we use complex media: tryptic soy media


enriched media - ✔✔complex media that contains additional "special"

nutrients/additives that are not commonly found in media


- least commonly used media


autoclave - ✔✔media is subjected to high-pressure steam at 121C at 15 lbs. of pressure

to sterilize and destroy any contaminants present


two major forms of media - ✔✔liquid and solid media


- media solidifies by adding agar (1.5%)


- agar is inert and incapable of growing by itself


what temperature do most bacterial organisms grow at? - ✔✔37C (98.6F)


colony - ✔✔A population of cells arising from a bacterial cell group or from a group of

attached bacterial cells


colony forming units (cfu) - ✔✔estimate the approximate number of bacteria in a liquid

broth culture


simple microscope - ✔✔contains a single magnifying lens and is similar to a magnifying

lens




Katelyn Whitman, All Rights Reserved © 2025 2

, compound microscope - ✔✔consists of two singles lenses arranged one in front of the

other so that the second lens magnifies the image produced by the first


illuminator - ✔✔light emits from this


condenser - ✔✔light passes through this and concentrates the light and focuses the light

onto the specimen


parfocal - ✔✔property of a microscope which allows objectives to be changed with

minimal or no refocusing


total magnification - ✔✔objective magnification (4X, 10X, 40X, 100X) x ocular

magnification (10X)


working distance - ✔✔distance between the objective and the object being viewed


depth of focus - ✔✔thickness of specimen that can be seen in focus at one time


resolving power - ✔✔the ability of the microscope to distinguish between two closely

adjacent points; measured in distance units


refractive index - ✔✔measure of how much the speed of light is reduced inside a

medium such as glass; the refractive index of air and glass are different enough so that

light waves change direction when they cross the interface from air to glass. The

necessitates that use of immersion oil with our 100X oil-immersion oil.


aseptic technique - ✔✔transferring without causing contamination to the culture



Katelyn Whitman, All Rights Reserved © 2025 3

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