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Microbiology Lab Exam # 1 Questions and Answers 100% Pass
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Microbiology Lab Exam # 1 Questions and Answers 100% Pass Considering the cultures used to inoculate each medium in this exercise, how many different microbial types should you expect to see in/on each medium? (1.4) - You should only have 1 if using good sterile technique. A successful aseptic ...

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  • January 17, 2025
  • 42
  • 2024/2025
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  • microbiology lab exam 1 questions and answers 10

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Microbiology Lab Exam # 1 Questions
and Answers 100% Pass

Considering the cultures used to inoculate each medium in this exercise, how many

different microbial types should you expect to see in/on each medium? (1.4) - ✔✔You

should only have 1 if using good sterile technique. A successful aseptic transfer will

have only micrococcus lutes growth- this would be a pure culture


You were asked to describe differences in appearance of growth in each culture, if

present. In which medium was this the most difficult to determine? What made it

difficult? (1.4) - ✔✔More difficult to see in broth because you can only see if growth

occurred on the top or bottom etc. Better in slant because you can observe different

growth patterns.


Which medium was most difficult for you to transfer from? Which medium was most

difficult for you to inoculate? Why? (1.4) - ✔✔Most people don't like working with

slants (solid)


If you got growth on sterile NA and NB slant rubes, why? (1.4) - ✔✔Possible

contamination include not heating the loop to orange-hot, not holding the open tubes

on an angle, and/or placing the cap on the table surface during the process.



Katelyn Whitman, All Rights Reserved © 2025 1

,Did you notice a difference in density (turbidity) of growth in NB tubes inoculated from

NB and NA slants? Possible reasons?( 1.4) - ✔✔Generally, more cells are transferred

from growth on a solid medium than from a broth culture. Therefore, broth cultures

made from growth on a solid medium will show greater turbidity than those inoculated

from a broth culture.


Did you notice a difference in density of growth on NA slants inoculated from NA

slants and NB? (1.4) - ✔✔Generally there will be denser growth on the slant inoculated

from the NA slant, because more cells are transferred from solid medium than broth.


Pure culture (1.4) - ✔✔When a culture ( a medium that contains living microbes)

contains a single species.


Broths (1.4) - ✔✔A medium used to grow microbes when fresh cultures or large

numbers of cells are required. Used for ID.


Agar Slant (1.4) - ✔✔A type of medium used to grow stock cultures that can be

refrigerated after incubation and maintained for several weeks.


Plated Media (1.4) - ✔✔A type of medium used for obtaining isolation of species,

differential testing, and quantifying bacterial densities.


Inoculating loops (1.4) - ✔✔An instrument used to inoculate a medium.


Inoculation (1.4) - ✔✔To introduce (cells or organisms) into a culture medium.




Katelyn Whitman, All Rights Reserved © 2025 2

,Using a pencil, draw a quadrant streak (1.5) - ✔✔Should look like this. You should also

rotate a little less than 90 Degrees each streak, and heat the loop so you can get good

results. Also, let the loop cool!!


Mixed Culture (1.5) - ✔✔A microbial culture consisting of two or more species.


What is generally the first step in identifying a microbial organism? (1.5) - ✔✔Obtaining

isolations of Individual colonies. The technique we used in class was the isolation

technique- the streak plate. Cells that have been sufficiently isolated will grow into

colonies, consisting only of the original cell type.


Colonies (1.5) - ✔✔Individual microbial cell type. They can also form from a pair, chain,

or cluster of cells.


Colony Forming Unit (CFU) (1.5) - ✔✔A more correct description of the colony origin


Zigzag Inoculation (1.5) - ✔✔A type of inoculation pattern used to when the sample

does not have a high enough cell density.


Quadrant streak technique: (1.5)




- how much space should you try to use?




- describe what each of the quadrants should look like



Katelyn Whitman, All Rights Reserved © 2025 3

, - describe order - ✔✔- maximize the space used




- Q1: confluent growth (colonies overlapping), max growth


- Q2: less growth, some separation


- Q3: even less growth


- Q4: isolated colonies




- flame, only go to culture once at the beginning, Q1, flame, Q2, flame, Q3, flame, Q4,

flame


Quadrant plate questions: (1.5)




- What if no overlap?


- too much overlap?


- plate with white and yellow colonies is what type of plate? - ✔✔- no overlap --> no

isolation


- too much overlap --> too much confluent growth




Katelyn Whitman, All Rights Reserved © 2025 4

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