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Microbiology Lab Practical #1 Review Questions and Answers 100% Pass $11.49
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Microbiology Lab Practical #1 Review Questions and Answers 100% Pass

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  • Microbiology Lab
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  • Microbiology Lab

Microbiology Lab Practical #1 Review Questions and Answers 100% Pass Bacterial Smear - Allows better visualization of specimens using microscopy. Heat Fixing - kills organisms, keeps organisms stuck to slide, allows the specimen to be stained more easily Aseptic Technique - preparing and handl...

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  • January 17, 2025
  • 5
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • Microbiology Lab
  • Microbiology Lab
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KatelynWhitman
Microbiology Lab Practical #1 Review
Questions and Answers 100% Pass


Bacterial Smear - ✔✔Allows better visualization of specimens using microscopy.


Heat Fixing - ✔✔kills organisms, keeps organisms stuck to slide, allows the specimen to

be stained more easily


Aseptic Technique - ✔✔preparing and handling sterile products in a manner that

prevents microbial contamination


Gram Staining Procedure - ✔✔1. Heat fix bacterial smear


2. Cover with Crystal Violet (Primary Stain) and wait 60 seconds


3. Rinse slide with distilled water until stain is removed


4. Cover with Gram's Iodine (Mordant) and wait for 60 seconds


5. Rinse with distilled water


6. 2-3 drops of Decolorizer (95% Ethanol), or until color is clear, rinse with distilled

water


7. Cover with Safranin (Counter Stain) and wait 45 seconds




Katelyn Whitman, All Rights Reserved © 2025 1

, 8. Rinse with distilled water


9. Blot dry with Bibulous paper, observe under oil immersion and record results


Capsule Stain Procedure - ✔✔1. Agitate broth culture


2. 2-3 loop fulls of broth culture added to slide and allow to air dry (NOT HEAT

FIXING)


3. Cover with Crystal Violet (Primary Stain) and wait for 2 minutes


4. Rinse with 20% Copper Sulfate Solution (Decolorizer and counter stain)


5. DO NOT RINSE WITH WATER


6. Air dry for a few minutes and DO NOT BLOT


Observe under oil immersion and record results


Why do you not heat fix during the capsule stain procedure? - ✔✔Heat will cause the

capsule to dislodge


Why do you not rinse with water during the capsule stain procedure? - ✔✔Water will

remove the capsule from the cell


Why do you not blot during the capsule stain procedure? - ✔✔Blotting will remove the

bacteria from the slide and/or distort the capsule




Katelyn Whitman, All Rights Reserved © 2025 2

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