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IMMUNOLOGY AND SEROLOGY PART 1 questions with verified answers

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Immunology and Serology

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Immunology And Serology

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Instelling Immunology and Serology
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IMMUNOLOGY AND SEROLOGY PART 1 questions
with verified answers
Primary Immunologic Reaction Ans✓✓-Combination of Antigen-Antibody
IRI = IF, RIA, IEA

Secondary Immunologic Reaction Ans✓✓-lattice formation
CAP = complement fixation, agglutination, precipitation

Tertiary Immunologic Reactions Ans✓✓-Ag-ab rxn is not visible but is detected by
the effect the reaction has on the tissues or cells
In vivo
Phagocytosis, opsonization, chemotaxis, immune adherence

Affinity Ans✓✓-How well the antibody fits to the shape of the antigen (goodness
of fit)

Avidity Ans✓✓-sum of all the attractive forces between an antigen and an
antibody

Agglutination Ans✓✓-Aggregation of particulate/insoluble test antigens

Precipitation Ans✓✓-Aggregation of soluble test antigens

7.0 Ans✓✓-pH used for routine lab testing

, 4-22C Ans✓✓-IgM are cold reacting with thermal range of

37C Ans✓✓-IgG are warm reacting with optimum temp of rxn at

15-60 mins Ans✓✓-Length of time of incubation of the coated particles with
coated serum

Direct agglutination Ans✓✓-Insoluble particle which is in suspension is reacted
with an Ab thus resulting to aggregation of particles

Direct immune Ans✓✓-Antibodies reactive with antigens on the native particle
Ex. A B antibodies

Direct nonimmune Ans✓✓-Lectins (phytohemagglutinins) are not antibodies
hence the direct hemagglutination of blood group O cells by anti-H lectin is
considered as

Blood typing, Widal, Weil-Felix, Hemagglutination inhibition Ans✓✓-Applications
of direct agglutination

Hemagglutination inhibition Ans✓✓-Measures the ability of soluble antigen to
inhibit the agglutination of antigen-coated red blood cells by antibodies

Antibodies + Ag on rbcs + Ag in Px sample = competition = no agglutination
Ans✓✓-Test principle of hemagglutination inhibition

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