100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
Biol 310 Exam 3 Verified Answers (2025)!! $15.99
Add to cart
Quickly navigate to
Preview
  2.  
  3. BIOL 310
  4.  
  5. BIOL 310

Exam (elaborations)

Biol 310 Exam 3 Verified Answers (2025)!!
 0 purchase
  • Course
  • BIOL 310
  • Institution
  • BIOL 310

BIOL 310 Study Material – Comprehensive & High-Quality Notes! Ace your BIOL 310 course with this well-organized and detailed study material! Perfect for students looking to save time, boost their grades, and understand key concepts quickly. What’s Included? Detailed Lecture Notes – C...

[Show more]

Preview 4 out of 33  pages

Document information

  • February 12, 2025
  • 33
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

Subjects

  • biol 310 exam 3 verified answers 2025

Written for

  • BIOL 310
  • BIOL 310

Seller

avatar-seller
Schoolflix

Reviews received

Content preview

Q/As




Biol 310 Exam 3 Verified Answers
(2025)!!



vesicular transport - Answers movement of lipids and proteins
between several cellualr components involves vesicular transport



shekman - Answers -ID'd machinery through yeast genetics

-screened for temperature sensitive, secretion defective yeast mutants

-categorized mutants by their "roadblock" phenotypes using transmission
electron microscopy

-identified broken gene

-"lots of vesicles" phenotype: sec; secretory defective



rothman: ER->golgi transit N-linked oligosaccharide processing -
Answers -endoglycosidase D:

-does nothing with Man8 (ER)

-cleaves with Man5 (Golgi)

-anti-VSV G and western blot used




https://www.stuvia.com/en-us/user/SCHOOLFLIX

, Q/As
rothman: is transport between cellular components possible after cell
homogenization - Answers -homogenize cells

-infected wild-type: N-acetyl glucosamine transferase

-infected mutat: no N-acetyl glucosamine added

-fusing uninfected wild type and infected mutant->n-acetyl glucosylated
VSV G protein

-repeat with biochemically fractionated cytoplasm, with mutations in other
glycosylation enzymes, and with chemical inhibitors (non-hydrolysable
GTP)



with nonhydrolysable GTP - Answers -an accumulation of distinct
classes of "coated vesicles"

1. suggested GTP hydrolysis is involved in the uncoating step

2. provided a source of biochemically enriched vesicles for analysis

-ARF associates with GEF at membrane

-ARF GTP recruits coat proteins

-ARF GDP triggers uncoating



vesicle budding basics - Answers -localized GEF recruits and
activated monomeric GTPase

-bound GTPase recruits cytosolic proteins

-coat proteins sculpt membrane and recruit cargo and snares

-types of coated vesicles: COPII, COPI, and clathrin




https://www.stuvia.com/en-us/user/SCHOOLFLIX

, Q/As
COPII vesicles - Answers -Sar1: another monomeric
GTPase+localized GEF (at membrane)

-inactive, soluble Sar1-GDP has hidden amphiphillic helix

-Sar1-GEF makes Sar1-GTP and associated with membrane (due to
exposed amphiphillic helix)

-binds to dimer Sec24 and Sec23 which binds to cargo and bends vesicle



sorting sequences - Answers -how are cargo proteins selected

-cytoplasmic sequences: transmembrane proteins

-luminal sequences: bind cargo-receptor protein)



self-assembling sec13/sec31 - Answers -coat assembly sculpts 50
nm vesicles

-coated vesicles detach from donor membrane

-then, with GTP hydrolysis, "tail" rtracts

-coat detaches



why must the coat come off - Answers -vesicles have cargo and
essential targeting proteins

-naked vesicles with exposed SNAREs fuse with next compartment

-sec23/sec24 act as GAP for Sar-1

-state of Sar-1 depends on relative levels of sec12 (Sar GEF) and Sec23
(GAP activity)

-GAP activity increases 10x after Sec13/31 binds->Sar1 is no longer near
Sec12 (GEF)


https://www.stuvia.com/en-us/user/SCHOOLFLIX

, Q/As


SNARE and Rab mechanism - Answers -Rab-GTP binds to Rab
effector on target membrane (tethering protein; encourages hydrolyzing)

-docking

-v-SNARE binds to trans-SNARE complex

-fusion

-Rab becomes GDP and binds to GDI (inhibitor) and cargo is released

-usually 1 v-SNARE and 3 t-SNAREs (need 4 alpha helices)

-Rabs accelerate fusion



thermodynamics of SNAREs - Answers -@1.5 nm, fusion is
energetically favorable

-clear other membrane proteins

-squeeze out H2O

-coiled the coiled-coil

-SNARE proteins provide a hydrophobic surface for formation of lipid bridge



after fusion of vesicle - Answers NSF and ATP required to uncoil
SNARE so v-SNAREs can be recycled back to donor membrane



how do v-SNARES go back to donor membrane - Answers -COPI
coated vesicle

-also brings back missorted ER resident proteins (like BiP0




https://www.stuvia.com/en-us/user/SCHOOLFLIX

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller Schoolflix. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $15.99. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

70113 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 15 years now

Start selling

Recently viewed by you

Other ·

(0)

SFM, Sport- en festivalmanagement

Summary ·

(0)

Energy Formulas GCSE 9-1 Physics

Presentation ·

(0)

S17 positieve gezondheid en preventie

Summary ·

(0)

Samenvatting EU Competition Law

Summary ·

(0)

Samenvatting psychomotoriek KBK

Package deal ·

(0)

WGU D220 Exam Latest Test Bank (2023)

Exam (elaborations) ·

(0)

BUS 5113 Discussion Assignment Unit 7
$15.99
  • (0)
Add to cart
Added