Polymerases/Restriction Enzymes (MB ASCP) Questions and Answers 100% correct

Polymerases/Restriction Enzymes (MB ASCP) Questions and Answers 100% correct α (Eukaryotic) DNA Polymerase Alpha Lagging strand synthesis during DNA replication. Found in Nucleus- Family B β (Eukaryotic) DNA Polymerase Beta DNA Repair mechanisms, and Replication restart at nicks and lesions Found in Nucleus Family X δ (Eukaryotic) DNA Polymerase Delta Primary DNA polymerase- synthesizes leading strand. Found in Nucleus- Family B ε (Eukaryotic) DNA Polymerase Epsilon mostly for repair but can substitute for Delta when needed. Found in Nucleus- Family B γ (Eukaryotic) DNA Polymerase Gamma mitochondrial replication only Found in Mitochondria- Family A Pol I (prokarytotic) DNA Polymerase 1 Lagging strand synthesis during DNA replication. 15-20 nucleotides added per second onto the RNA Primer Pol II DNA Polymerase 2 DNA Repair mechanisms, and Replication restart at nicks and lesions Can interact with POL III and holoenzymes. Pol III DNA Polymerase 3 Primary DNA polymerase- synthesizes leading strand. major contributor to holoenzyme activity. 1000+ nucleotides per second RNA Pol I Sythesizes rRNA needed for translation RNA Pol II Synthesizes mRNA Gene transcription RNA Pol III Synthesizes tRNA, and snRNA Needed for protein translation RE I Type 1 Restriction Enzymes nuclease and methylase activity- recognizes specific sites 4-6 bp separated by 6-8 bp and contains methylated adenines cuts up to 1000 bp away from recognition site. RE II Type 2 Restriction Enzymes Recognition sites are palindromic and cut is at or near the recognition site. cut methods can differ leaving "sticky Ends" or "blunt ends" RE III Type 3 Restriction Enzymes Resemble Type one enzymes-recognize sites that are asymmetrical cuts 24-26 bp away from recognition site 3' side RE IV Type 4 Restriction Enzymes Resemble Type one enzymes-recognize sites that are asymetrical cuts 10 bp away from recognition site 3' side