Uni 6 Assignment One: Investigative Study Proposal and Literature Review
Learning Aim: The primary objective of Uni 6 Assignment One is to equip students with the skills necessary to create a comprehensive proposal for an investigative study, along with conducting a thorough literature review. T...
Unit 6: Investigative Project
Learning Aim: to create a proposal for an investigative study, conduct a
literature review.
Fatima Kalaf
ID: 40180037
, Proposal
The purpose of this experiment is to discuss and determine the effects of different
concentrations of methanol on heart rate using flea’s daphnia. A typical light microscope
makes Daphnia plainly visible at low magnification. The heart rate, which can reach 300
beats per minute, can be measured, and quantified under a variety of circumstances, such
as changing the water's temperature, or the type and quantity of chemicals added. The
strategy offers an intriguing method for examining how various compounds affect an
animal's metabolic process. Daphnia is a poikilothermic organism, which implies that the
environment's temperature has a direct impact on both its body temperature and metabolic
rate. The rate at which the heart beats change in response to changes in metabolic rate
(cardiac frequency). methanol beverages cause Daphnia's heart rate to decrease. The heart
rate of Daphnia lowers with an increase in alcohol concentration.
Hypothesis As the concentration of methanol increases, the heart rate of daphnia
decreases
Equipment
- Few daphnia - to be able to complete the experiment we need daphnia to the experiment
on.
- Microscopes slides (concavity slides are ideal) - we need microscopes slides to hold the
daphnia under the microscope.
- 3 plastic pipettes - each pipette will be used for a different concentration of alcohol on the
daphnia.
- A microscope - to exam the daphnia's heart rate
- Beaker - to place the daphnia.
- Distilled water - so the daphnia don't die.
- 1% caffeine solution
- 1% alcohol solution
- Stopwatch - to be to exam how fast or slow the heart rate of daphnia is for each alcohol
concentrations.
- Counter - to count the daphnia heart rate.
Method
15 ml of distilled water was added to a small beaker.
A micropipette was used to transfer the daphnia into the beaker. Using the pipette one drop of
distilled water was added to the microscope slide, the daphnia was then transferred to the
microscope slide. The slide with daphnia was then observed under the microscope set.The number
of heart beat that occurred within 20 seconds was counted, while I was counting, the otter partner
timed for 20 seconds on the stop watch.
Daphnia's heartbeat is quite quick, therefore mark dots in the form of the letter S on a piece of
paper to count the beats. Convert the number of dots to beats per minute to determine the heart
rate. Create a table to store the findings.
The amount of heartbeat for 20 seconds was then recorded, 1 drop of alcohol was then added to the
microscope slide using a pipette. Daphnia was observed and the number of heart beats were
counted again for 20.
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