MIBO 3500 Final Questions with correct answers

For Escherichia coli, what is the genus and species name? CORRECT ANSWER genus: Escherichia species: Escherichia coli What dutch merchant was the first to us a microscope to observe since-celled microbes? What did he call these single-celled microbes? CORRECT ANSWER Leeuwenhoek, Animalcules The periplasmic space refers to what? CORRECT ANSWER Space between inner membrane and outer membrane True or False: The key element in Pasteur's experiments disproving the theory of Spontaneous Generation was his use of flasks having a swan neck that trapped contaminating microbes in the air. CORRECT ANSWER True True or False: Certain microbes in the soil accelerate the oxidation of metal sulfides in groundwater from abandoned, subsurface mines, making the water extremely acidic. CORRECT ANSWER True The classification of all living things into three domains is based upon the analysis of: CORRECT ANSWER 16S RNA sequence True or False: As the wavelength of electromagnetic radiation increases, its penetrating ability and resolving power increases. CORRECT ANSWER False The staining technique to use on sputum from a suspected case of tuberculosis is: CORRECT ANSWER Acid-fast stain This term in microscopy is defined as the minimum distance between two objects where they still appear separate. CORRECT ANSWER Resolution Briefly explain how dark-field condensers creates a dark background for darkfield microscopy. CORRECT ANSWER The objective picks up only scattered light from a specimen. If light is un-scattered then no light enters the objective and the background will appear dark due to the hollow c