ASCP Hematology
Dohle bodies - ✅✅-Light blue patches in cytoplasm of neutrophils. Infection,
burns
✅✅-O2 transport. Produced in BM
Erythrocytes -
Mean Corpuscular Hemoglobin (MCH) formula - ✅✅-(HGBx10)/RBC
✅✅
Mean Corpuscular Hemoglobin Concentration (MCHC) formulao -
-(HGBx100)/HCT
Granulocytes -✅✅-Defense against bacterial infection. Produced in BM
Lymphocytes - ✅✅-Cellular and humoral immunity. Produced in lymphoid
tissue
✅✅-Coagulation. Produced in BM
Platelets -
WBC RR - ✅✅-4.5-11.5 x10^3 / microL
RBC RR - ✅✅-M: 4.6-6x10^6 / microL
F: 4-5.4x10^ / microL
HGB RR - ✅✅-M: 14-18 g/dL
F: 12-15 g/dL
HCT RR - ✅✅-M: 40-54%
F: 35-49%
MCV RR - ✅✅-80-100 fL
MCH RR - ✅✅-27-31 pg
MCHC RR - ✅✅-32-36%
PLT RR - ✅✅-150-450x10^3 microL
,MCV first 5 days - ✅✅-Macrocytes. Higher in preterm infants
Retic % -✅✅-newborns increased polychromasia
Seg % - ✅✅-50-70
Band % - ✅✅-0-5
Lymph newborn - ✅✅-few benign immature B cells may be seen
common myeloid progenitor - ✅✅-differentiate into erythrocytes, platelets
(megaK), granulocytes (basophils, eosinophil, neutrophil), and monocytes
common lymphoid progenitor (CLP) - ✅✅-T-lymph, NK cell, B-lymph
Multipotential progenitor cell - ✅✅-Common myeloid progenitor or common
lymphoid progenitor
Erytheopoiesis 1-2 month gestation - ✅✅-yolk sac and aorta-gonads
mesonephros region (gower I/II and portland)
Erythropoiesis 3-6 month gestation - ✅✅-liver (primary), spleen
Erythropoiesis 7 month of gestation-4 years - ✅✅-Bone marrow (all marrow
active)
Erythropoiesis adult - ✅✅ -bone marrow (pelvis, vertebrae, ribs, sternum, skull.
Shafts of long bones filled with fat-may reactivate to compensate for anemia-
liver and spleen may reactivate if that fails to keep up with demand)
Rubriblast/pronormoblast - ✅✅-14-24 um
N:C ratio 8:1
Royal blue cytoplasm. Fine chromatin
1-2 nucleoli
Normally confined to bone marrow.
, Prorubricyte/basophilic normoblast - ✅✅ -12-17 um
N:C ratio 6:1
Chromatin is coarser with slightly visible parachromatin
Nucleoli not visible
Normally confined to bone marrow.
Rubricyte/polychromatophilic normoblast - ✅✅ -10-15 um. N:C ratio 4:1.
Cytoplasm is polychromatophilic due to hemoglobin production. Chromatin is
clumped with distinct areas of parachromatin. Last stage to divide. Normally
confined to bone marrow.
Metarubricyte/orthochromic normoblast - ✅✅-8-12 um
N:C ratio 1:2
Nucleus is pyknotic
Last nucleated stage
Normally confined to bone marrow.
Reticulocyte/Polychromatophilic erythrocyte - ✅✅ -7-10 um
No nucleus. Cytoplasm is diffusely basophilic (bluish tinge)
Reticulum seen with supravital stain. 0.5%-1.5% of RBCs in adult peripheral
blood.
Mature erythrocyte - ✅✅ -7-8 um
Biconcave disk. Reddish-pink cytoplasm with area of central pallor 1/3 diameter
of cell.
Megaloblastic anemia cause - ✅✅-vitamin b12 or folic acid deficiency (ex
pernicious anemia)
Megaloblastic characteristics -✅✅ -Nucleus lags behind cytoplasm in
maturation. cells grow larger without dividing. Oval macrocytes
✅✅
Iron deficiency characteristics - -cytoplasm lags behind nucleus in
maturation due to inadequate iron for hgb synthesis. Microcytic, hypochromic
RBCs
Hemoglobin A - ✅✅-2 alpha + 2 beta, >95% adults, 20% newborn
Dohle bodies - ✅✅-Light blue patches in cytoplasm of neutrophils. Infection,
burns
✅✅-O2 transport. Produced in BM
Erythrocytes -
Mean Corpuscular Hemoglobin (MCH) formula - ✅✅-(HGBx10)/RBC
✅✅
Mean Corpuscular Hemoglobin Concentration (MCHC) formulao -
-(HGBx100)/HCT
Granulocytes -✅✅-Defense against bacterial infection. Produced in BM
Lymphocytes - ✅✅-Cellular and humoral immunity. Produced in lymphoid
tissue
✅✅-Coagulation. Produced in BM
Platelets -
WBC RR - ✅✅-4.5-11.5 x10^3 / microL
RBC RR - ✅✅-M: 4.6-6x10^6 / microL
F: 4-5.4x10^ / microL
HGB RR - ✅✅-M: 14-18 g/dL
F: 12-15 g/dL
HCT RR - ✅✅-M: 40-54%
F: 35-49%
MCV RR - ✅✅-80-100 fL
MCH RR - ✅✅-27-31 pg
MCHC RR - ✅✅-32-36%
PLT RR - ✅✅-150-450x10^3 microL
,MCV first 5 days - ✅✅-Macrocytes. Higher in preterm infants
Retic % -✅✅-newborns increased polychromasia
Seg % - ✅✅-50-70
Band % - ✅✅-0-5
Lymph newborn - ✅✅-few benign immature B cells may be seen
common myeloid progenitor - ✅✅-differentiate into erythrocytes, platelets
(megaK), granulocytes (basophils, eosinophil, neutrophil), and monocytes
common lymphoid progenitor (CLP) - ✅✅-T-lymph, NK cell, B-lymph
Multipotential progenitor cell - ✅✅-Common myeloid progenitor or common
lymphoid progenitor
Erytheopoiesis 1-2 month gestation - ✅✅-yolk sac and aorta-gonads
mesonephros region (gower I/II and portland)
Erythropoiesis 3-6 month gestation - ✅✅-liver (primary), spleen
Erythropoiesis 7 month of gestation-4 years - ✅✅-Bone marrow (all marrow
active)
Erythropoiesis adult - ✅✅ -bone marrow (pelvis, vertebrae, ribs, sternum, skull.
Shafts of long bones filled with fat-may reactivate to compensate for anemia-
liver and spleen may reactivate if that fails to keep up with demand)
Rubriblast/pronormoblast - ✅✅-14-24 um
N:C ratio 8:1
Royal blue cytoplasm. Fine chromatin
1-2 nucleoli
Normally confined to bone marrow.
, Prorubricyte/basophilic normoblast - ✅✅ -12-17 um
N:C ratio 6:1
Chromatin is coarser with slightly visible parachromatin
Nucleoli not visible
Normally confined to bone marrow.
Rubricyte/polychromatophilic normoblast - ✅✅ -10-15 um. N:C ratio 4:1.
Cytoplasm is polychromatophilic due to hemoglobin production. Chromatin is
clumped with distinct areas of parachromatin. Last stage to divide. Normally
confined to bone marrow.
Metarubricyte/orthochromic normoblast - ✅✅-8-12 um
N:C ratio 1:2
Nucleus is pyknotic
Last nucleated stage
Normally confined to bone marrow.
Reticulocyte/Polychromatophilic erythrocyte - ✅✅ -7-10 um
No nucleus. Cytoplasm is diffusely basophilic (bluish tinge)
Reticulum seen with supravital stain. 0.5%-1.5% of RBCs in adult peripheral
blood.
Mature erythrocyte - ✅✅ -7-8 um
Biconcave disk. Reddish-pink cytoplasm with area of central pallor 1/3 diameter
of cell.
Megaloblastic anemia cause - ✅✅-vitamin b12 or folic acid deficiency (ex
pernicious anemia)
Megaloblastic characteristics -✅✅ -Nucleus lags behind cytoplasm in
maturation. cells grow larger without dividing. Oval macrocytes
✅✅
Iron deficiency characteristics - -cytoplasm lags behind nucleus in
maturation due to inadequate iron for hgb synthesis. Microcytic, hypochromic
RBCs
Hemoglobin A - ✅✅-2 alpha + 2 beta, >95% adults, 20% newborn
