BCH210 TESTED QUESTIONS WITH REVISED ANSWERS – UPDATED

BCH210 TESTED QUESTIONS WITH REVISED ANSWERS – UPDATED cross linker can be used in what techniques - Answer-stabilize X ray structure limitation of BiOID - Answer-can only proximate, maybe the proteins are just passing by the POI beads connect to what in BiOid - Answer-biotin released by ligase what is PDI - Answer-protein disulfide isomerase a kind of chaperon, isomerase structure of DE at pKa, why - Answer-C=O no - charge because protonated are cofactors proteins - Answer-no what is heme - Answer-prothetic organic cofactors, bind tightly, shuttle O2 why N S are beta turn AA - Answer-their R group can be modified for PTM describe zinc finger - Answer-is a motif, zinc is the ligand, bind 2 cys and 2 his, for stabilizing and DNA recognition chaperon binds to where and has what property - Answer-hydrophobic, amphipathic two sites of enzyme - Answer-active-binds substrate allosteric- binds to other for conformational change basic and acid are hydrophilic - Answer-si imidazole is an aromatic ring - Answer-si mass spectrometry can only be used on where - Answer-known peptides Dialysis prefer what proteins - Answer-large affinity chromatography, what is the bond between resin and ligand, ligand and POI - Answer-covalent noncovalent