Lab Practical Exam 1 Biology 2420
Questions and Correct Answers
Hand Hygiene (Hand Washing) ✅Lathers all surfaces of wrists, hands, and fingers
producing friction, for at least 20 (twenty) seconds, keeping hands lower than the
elbows and the fingertips down. Rinse all surfaces of wrists, hands, and fingers, keeping
hands lower than the elbows and the fingertips down.
Aseptic Technique ✅-precautions taken to prevent contamination of a surgical wound
- Prevents the release of infectious agents from cultures into the environment
Heat fixation ✅This kills bacteria, makes them adhere to the slide, and coagulates
cytoplasm proteins to make them more visible. ( May distort cells)
Why do we Stain? ✅- Increase contrast where cells are normally transparent
- Determine cell size, morphology and arrangement.
What types of hazards should you protect yourself from in the laboratory? ✅Spills
Splattering
Flame
Corrosive chemicals
Sharp objects
Who and/ or what is protected by lab safety protocols? ✅- students doing lab activities
- lab instructors and technical assistants
- anyone who walks into the lab
- the physical lab space and the equipment housed within the lab
Which of the following represent important safety procedures for microbiology students?
✅- Wash your hands before and after laboratory procedures.
- Disinfect your lab bench at the beginning and end of the lab period.
- Take extra care around the flame of a Bunsen burner by tying up hair and keeping it
from combustibles.
Which of the following are potential hazards which you might encounter in the
laboratory? ✅-Infectious microorganisms
-open flames
-hot surfaces
-corrosive chemicals
-sharp tools or broken glass.
-gamma radiation
- magnetic fields
, In which circumstance is food and/ or beverage allowed in the laboratory? ✅never
A disinfectant is used on your work surface? ✅-before the beginning of lab procedures,
-after all work is complete,
-after any spill of live organisms.
- All of the answers are correct
Using a cell phone in lab is permissible since it can be used for timing experiments.
✅False
Which of the following items may be on the laboratory bench top while doing lab work?
✅-Cultures
- equipment items specifically needed to complete the lab activity
- pencil or pen
What should be done to prepare or deal with a laboratory emergency? ✅-Know the
location of safety equipment such as the fire extinguisher
- Call 911 if an emergency occurs
- Take antibiotics regularly before attending class in case of spills
- Conduct all lab procedures under a fume hood
Which of the following are reasons for washing hands before and after lab activities?
✅- Hands may be a source of contamination to the lab experiments.
- Hands may become contaminated with infectious bacteria during lab experiments.
- Hands may become stained during lab.
- Hands may be contaminated with irritating chemicals during experiments.
Place the following steps of the hand washing procedure in the correct order. ✅1)
Thoroughly wet hands in running water.
2) Apply soap.
3) Lather for at least 20 seconds by
rubbing hands together, paying attention to the back of hands, nails, and between
fingers.
4) Rinse thoroughly in running water.
5) Dry hands thoroughly and use a towel to turn off the faucet.
How long should hands be lathered while washing? ✅20 seconds
Paper towels should be discarded in a biohazard container after washing hands.
✅False
Questions and Correct Answers
Hand Hygiene (Hand Washing) ✅Lathers all surfaces of wrists, hands, and fingers
producing friction, for at least 20 (twenty) seconds, keeping hands lower than the
elbows and the fingertips down. Rinse all surfaces of wrists, hands, and fingers, keeping
hands lower than the elbows and the fingertips down.
Aseptic Technique ✅-precautions taken to prevent contamination of a surgical wound
- Prevents the release of infectious agents from cultures into the environment
Heat fixation ✅This kills bacteria, makes them adhere to the slide, and coagulates
cytoplasm proteins to make them more visible. ( May distort cells)
Why do we Stain? ✅- Increase contrast where cells are normally transparent
- Determine cell size, morphology and arrangement.
What types of hazards should you protect yourself from in the laboratory? ✅Spills
Splattering
Flame
Corrosive chemicals
Sharp objects
Who and/ or what is protected by lab safety protocols? ✅- students doing lab activities
- lab instructors and technical assistants
- anyone who walks into the lab
- the physical lab space and the equipment housed within the lab
Which of the following represent important safety procedures for microbiology students?
✅- Wash your hands before and after laboratory procedures.
- Disinfect your lab bench at the beginning and end of the lab period.
- Take extra care around the flame of a Bunsen burner by tying up hair and keeping it
from combustibles.
Which of the following are potential hazards which you might encounter in the
laboratory? ✅-Infectious microorganisms
-open flames
-hot surfaces
-corrosive chemicals
-sharp tools or broken glass.
-gamma radiation
- magnetic fields
, In which circumstance is food and/ or beverage allowed in the laboratory? ✅never
A disinfectant is used on your work surface? ✅-before the beginning of lab procedures,
-after all work is complete,
-after any spill of live organisms.
- All of the answers are correct
Using a cell phone in lab is permissible since it can be used for timing experiments.
✅False
Which of the following items may be on the laboratory bench top while doing lab work?
✅-Cultures
- equipment items specifically needed to complete the lab activity
- pencil or pen
What should be done to prepare or deal with a laboratory emergency? ✅-Know the
location of safety equipment such as the fire extinguisher
- Call 911 if an emergency occurs
- Take antibiotics regularly before attending class in case of spills
- Conduct all lab procedures under a fume hood
Which of the following are reasons for washing hands before and after lab activities?
✅- Hands may be a source of contamination to the lab experiments.
- Hands may become contaminated with infectious bacteria during lab experiments.
- Hands may become stained during lab.
- Hands may be contaminated with irritating chemicals during experiments.
Place the following steps of the hand washing procedure in the correct order. ✅1)
Thoroughly wet hands in running water.
2) Apply soap.
3) Lather for at least 20 seconds by
rubbing hands together, paying attention to the back of hands, nails, and between
fingers.
4) Rinse thoroughly in running water.
5) Dry hands thoroughly and use a towel to turn off the faucet.
How long should hands be lathered while washing? ✅20 seconds
Paper towels should be discarded in a biohazard container after washing hands.
✅False
