MLS ASCP – Chemistry || All Correct.
holminum oxide correct answers best suited for verifying wavelength
dispersion from 2nd order spectra correct answers most common cause of stray light, causes loss
of linearity at high-analyte concentration
glycolysis correct answers the conversion of glucose or other hexoses into lactate or pyruvate
heparin tube correct answers tube used for blood gas
absorbance correct answers proportional to the inverse log of transmittance. directly proportional
to concentration
spectrophotometry correct answers chemical rxn produces colored substance that absorbs
specific wavelength, most common
atomic absorption spectrophotometry correct answers measures light absorbed by ground-state
atoms, produces specific for analyte. very sensitive and used to find trace materials
fructosamine correct answers will monitor short term glycemic changes
lactic acid, ammonia, and blood gases correct answers chilling is required for what blood tube
collections?
total protein
LD
Ca2+ correct answers these will be increased in plasma
K+
phospate
glucose
CK
triglycerides
bicarbonate
ALP
albumin
AST correct answers these will be increased in serum
fluorometry correct answers atoms absorb light of specific wavelength and emit light of longer
wavelength. 90 degrees, more sensitive than colormetry. used to measure drugs and hormones
chemiluminescence correct answers chemical rxn produces light,extremely sensitive and is used
in immunoassys
turbidity correct answers used to measure proteins in urine and in CSF
, nephelometry correct answers similar to turbidity but light is measured at an angle
G6PD on the amino-terminal valine of the beta chain correct answers A1c is used to measure
what?
boiling point correct answers the elution orders of volatiles is based upon what?
70-99 correct answers fasting glucose range
>60 correct answers HDL range
<100 correct answers LDL range
<150 correct answers triglyceride range
6.4-8.3 correct answers total protein range
3.5-5 correct answers albumin range
osteoporosis correct answers high urine and serum n-telopeptide type 1 collagen
albumin > alpha-1 globulin >alpha-2 > beta > gamma correct answers order of protein
electrophoresis
gamma region correct answers Bence-jones proteins migrate to which region in electrophoresis?
Pheylkentonuria (PKU) correct answers deficiency of an enzyme that converts phenylaline to
tyrosine. Causes retardation and mousy odor urine. Use Guthrie bacterial inhibition assay to
confirm. all newborns are screened
Tyrosinemia correct answers disorder of tyrosine catabolism. Causes liver and kidney disease.
diagnosed by MS
Alkaptonuria correct answers deficiency of the enzymes needed in the metabolism of tyrosine
and phenylaline. Build up of homogentisic acid. Black urine and darkening of tissues. Hip and
back pain. diagnosed by gc/ms
maple syrup urine disease correct answers Enzyme deficiency leading to the build up of leucine,
isoleucine, and valine. Burnt sugar urine and breath. Failure to thrive. Mental retardation,
acidosis, seizures, coma and death. Diagnosed by modified Guthrie and MS
Homocystinuria correct answers deficiency in the enzyme needed to metabolize methionine.
Buildup of methionine and homocystine. Causes osteoporosis, dislocated lenses, mental
retardation, and thrombolic events. Diagnosed by modified Guthrie and MS
holminum oxide correct answers best suited for verifying wavelength
dispersion from 2nd order spectra correct answers most common cause of stray light, causes loss
of linearity at high-analyte concentration
glycolysis correct answers the conversion of glucose or other hexoses into lactate or pyruvate
heparin tube correct answers tube used for blood gas
absorbance correct answers proportional to the inverse log of transmittance. directly proportional
to concentration
spectrophotometry correct answers chemical rxn produces colored substance that absorbs
specific wavelength, most common
atomic absorption spectrophotometry correct answers measures light absorbed by ground-state
atoms, produces specific for analyte. very sensitive and used to find trace materials
fructosamine correct answers will monitor short term glycemic changes
lactic acid, ammonia, and blood gases correct answers chilling is required for what blood tube
collections?
total protein
LD
Ca2+ correct answers these will be increased in plasma
K+
phospate
glucose
CK
triglycerides
bicarbonate
ALP
albumin
AST correct answers these will be increased in serum
fluorometry correct answers atoms absorb light of specific wavelength and emit light of longer
wavelength. 90 degrees, more sensitive than colormetry. used to measure drugs and hormones
chemiluminescence correct answers chemical rxn produces light,extremely sensitive and is used
in immunoassys
turbidity correct answers used to measure proteins in urine and in CSF
, nephelometry correct answers similar to turbidity but light is measured at an angle
G6PD on the amino-terminal valine of the beta chain correct answers A1c is used to measure
what?
boiling point correct answers the elution orders of volatiles is based upon what?
70-99 correct answers fasting glucose range
>60 correct answers HDL range
<100 correct answers LDL range
<150 correct answers triglyceride range
6.4-8.3 correct answers total protein range
3.5-5 correct answers albumin range
osteoporosis correct answers high urine and serum n-telopeptide type 1 collagen
albumin > alpha-1 globulin >alpha-2 > beta > gamma correct answers order of protein
electrophoresis
gamma region correct answers Bence-jones proteins migrate to which region in electrophoresis?
Pheylkentonuria (PKU) correct answers deficiency of an enzyme that converts phenylaline to
tyrosine. Causes retardation and mousy odor urine. Use Guthrie bacterial inhibition assay to
confirm. all newborns are screened
Tyrosinemia correct answers disorder of tyrosine catabolism. Causes liver and kidney disease.
diagnosed by MS
Alkaptonuria correct answers deficiency of the enzymes needed in the metabolism of tyrosine
and phenylaline. Build up of homogentisic acid. Black urine and darkening of tissues. Hip and
back pain. diagnosed by gc/ms
maple syrup urine disease correct answers Enzyme deficiency leading to the build up of leucine,
isoleucine, and valine. Burnt sugar urine and breath. Failure to thrive. Mental retardation,
acidosis, seizures, coma and death. Diagnosed by modified Guthrie and MS
Homocystinuria correct answers deficiency in the enzyme needed to metabolize methionine.
Buildup of methionine and homocystine. Causes osteoporosis, dislocated lenses, mental
retardation, and thrombolic events. Diagnosed by modified Guthrie and MS
