PEARSON (PEARSON) • BTEC 3320
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Quiz 6 
Which of these changes to protein purification using an AKTA would most likely be 
classified as minor change? 
The flow rate was increased 25% higher. 
The buffer that is used in the mobile phase was replaced with a different weak acid 
The set number of times the column can be washed and reused is changed from 20 to 
25 reuses. 
The tempeature setting for the column was decrease 25% from the earlier settings. 
The detector was replaced with a newer model that is more sensitive in det...