HTL (Histotechnologist) Bundle Set

Alcian Blue demonstrates - Answer Acid mucins ATPase Enyme Histochemistry demonstrates - Answer ATPase in muscles Bielschowsky's Silver Technique demonatrates - Answer Dendrites, axons, neurofibrils Brown & Brenn gram stain demonstrates - Answer bacteria Congo red demonstrat...

The primary purpose of fixation is - Answer To stop enzymatic action Which fixative contains picric acid, formalin, and acetic acid? - Answer Bouin The volume of fixative should exceed the volume of the tissue by - Answer 15-20 times Tissue must be washed in water after fixation in - ...

Acetic Acid - Answer Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the nucleoproteins and fix the nuclei. Acetic Acid - Answer Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. Use...

What is the most important factor that would contribute to tissue firmness when cutting on the cryostat? - Answer Lipid and water content of tissue If you are making 1000 ml of a 0.25% acetic acid solution. Which instrument would you use to transfer the acetic acid? - Answer 5ml pipette If...

used to determine the origin of a tumor, prognosis and treatment. - Answer Immunohistochemistry is a host protein (immunoglobulin) produced in response to the presence of foreign molecules, organisms, or other agent in the body. - Answer antibody Immunoglobulins are proteins produced by ...

Endoplasmic Reticulum - Answer H&E basophilic staining within cytoplasm is due to what cellular structure? Pink - Answer Cytoplasm will show up what color on an H&E? 20 - Answer Proteins are made up of combinations of how many amino acids? Carboxyl group, side group - Answer All...

Dehydrating Agents - Answer Alcohol, dioxane, acetone, tetrahydrofuran. Clearing Agents - Answer Xylene, toluene, benzene, acetone, chloroform, cedarwood oil, tetrahydrofuran, dioxane, limonene, aliphatic hydrocarbons. Universal Solvent - Answer Solvents that perform both the dehydration...

Polarizing Microscope - Answer Microscope used to identify crystals, talc, silica, or urates. Birefringence - Answer Transmitting light unequally in different directions- Phase-Contrast Microscope - Answer Microscope used to examine unstained objects. Not routinely used in histopath labs...

Cresyl Echt Violet (Luna) method - Answer Nissl substances (basophilic) dyed, alcohol used to differentiate. Cresyl Echt Violet (Vacca) method - Answer Nissl substances (basophilic) dyed, acetic acid used to differentiate. Bodain Method for Nerve Fibers - Answer Protargol (silver solutio...

Too much blade tilt causes this:? - Answer Chatter Chatter may be caused by? - Answer Cutting too rapidly Naphthol AS-D chloroacetate esterase stains are rare among enzyme techniques since it may be used on these type of sections? - Answer Formalin fixed paraffin

Acetic Acid - Answer Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the nucleoproteins and fix the nuclei. Acetic Acid - Answer Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. U...

Cryptococcus neoformans - Answer a fungus that can be demonstrated with various stains for acidic mucins is the P.H. should be modified on the 4.6 stain - Answer ATPase stains done on a muscle biopsy at P.H.9.4,4.6 & 4.3.The slides for P.H.9.4 & 4.3 show good differentiation of the type 1 &...

This is a stain for acid mucopolysaccharides and sialomucins. - Answer Alcian blue This stains reacts with cations and is used to find calcium in tissue. - Answer Alizaran Red S This stains uses ferric ions to demonstrate mucopolysaccharides and glycoproteins. Must be done at a low pH. - A...

Glycogen is most abundant in - Answer Liver, heart (cardiac muscle cells), womb Staining reactions for glycogen: - Answer PAS (+), diastase (sensitive), Bauer-feulgen (+), Best carmine (+) Which two glycogen fixatives preserves the most glycogen? - Answer Bouins & Rossman's Other gly...