AAB immunology Fully solved 2023/2024 verified to pass
AAB immunology Fully solved 2023/2024 verified to passThis DNA amplification method amplifies the nucleic acid used as a probe? - correct answer Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? - correct answer DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? - correct answer Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? - correct answer Better specificity How does Ligase Chain Reaction differ from Polymerase Chain Reaction? - correct answer This method uses a thermostable ligase to join to probes which are then amplified by standard PCR cycling. Why was Ligase Chain Reaction originally developed? - correct answer To detect point mutation. What do positive controls ensure during PCR testing? - correct answer That the enzyme is active, the buffer is optimal, the primers are priming the correct sequence, and that the thermal cycler is working properly. What is a reagent blank con
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