MB(ASCP) Exam Practice Latest Update 2024 Graded A

MB(ASCP) Exam Practice Latest Update 2024 what does topoisomerase do? - Answer ️️ -breaks phosphate backbone to "un" supercoil DNA Another name for Topoisomerase II - Answer ️️ -Gyrase Helicase - Answer ️️ -Un"zips" DNA at replication fork What makes up the primosome? - Answer ️️ -1. primase 2. helicase 3. DNA C NASBA stands for... - Answer ️️ -nucleic acid sequence based amplification NASBA - Answer ️️ -RNA is target RNA--cDNA Reverse transcriptase (RNA--cDNA) followed by DNA dependent polymerase (cDNA--RNA) RNAse H degrades original RNA template Evaluated gene EXPRESSION - Answer ️️ -RNA ratio for organic extraction - Answer ️️ -Phenol (50): Chloroform (49): isoamyl alcohol (1) phenol - Answer ️️ -denatures proteins, dissolves lipids chloroform - Answer ️️ -assists in dissolving remaining lipids, gives a clear interphase between aqueous phase Long term storage of RNA - Answer ️️ -70% EtOH at -70 C ratio for 28S to 18S subunits - Answer ️️ -2:1 (can see this in a gel) A260 - Answer ️️ -nucleic acids A280 - Answer ️️ -protein A230 - Answer ️️ -organic compounds, phenol A270 - Answer ️️ -phenol optical density of ds DNA - Answer ️️ -50 ug/ml optical density ssDNA - Answer ️️ -33 ug/ml optical density RNA - Answer ️️ -40 ug/ml DNA concentration is calculated: - Answer ️️ -A260 x dilution factor x 50 ug/ml RNA concentration = - Answer ️️ -A260 x dilution factor x 40 ug/ml DNA B - Answer ️️ -helicase DNA G - Answer ️️ -primase Processivity - Answer ️️ -# of dNTPs that a polymerase can incorporate continuously without dissociating from primer template fidelity - Answer ️️ -error rate of polymerase star activity - Answer ️️ -RE : additional cleavages at imperfect recognition sites synthesis of DNA is in what direction: - Answer ️️ -5'--3' add to 3' -OH Taq DNA polymerase - Answer ️️ -thermus aquaticus thermostable used in amplification 3 termination sequences - Answer ️️ -UGA UAA UAG Huntingtons Disease (genetic basis) - Answer ️️ -Autosomal Dominant Chr 4p1