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Exam (elaborations)

GPC Questions and Answers

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GPC Questions and Answers How can streptococci be differentiated from staphylococci? ANSWER Using a catalase test staph is positive strep is negative How would staphylococcus react on a catalase test? ANSWER Form bubbles, positive How would streptococcus react in a catalase test? ANSWER No reaction. Negative After colonial morphology, gram stain and a positive catalase test, what is the next step in identification? ANSWER Either tube coagulation or latex agglutination What does a positive coagulase test on a catalase positive GPC indicate? ANSWER The organism is Staphylococcus aureus What does a negative coagulase test on a catalase positive GPC indicate? ANSWER If the culture is a urine sample from a young female, more testing needs to be done Novobiocin What organism is identified by its resistance to novobiocin? ANSWER Staphylococccus saprophyticus What is staphylococcus saprophyticus associated with? ANSWER urinary tract infections in young women Your GPC tests positive for catalase, negative for latex agglutination and is resistant to novobiocin. What do you report? ANSWER Staphylococcus saprophyticus Describe the appearance of staphylococcus colonies vs streptococcus colonies ANSWER staphylococcus is usually white and opaque streptococcus is grey and translucent, however, enterococcus is very dense and can look white Your GPC tests negative for catalase. What is the next step? ANSWER Identify hemolysis

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