Exam (elaborations)
Electrophoresis Questions With Complete Solutions
- Course
- Institution
Electrophoresis Questions With Complete Solutions
[Show more]
Content preview
Electrophoresis Questions With Complete SolutionsElectrophoresis Right Ans - A separation technique that is based on the
movement of charged particles in an electric field.
Electromigration Right Ans - At any given pH, charged molecules may exist
as anions (move to anode) or cations (move to cathode)
Electromotive Force Right Ans - Basis of Electrophoresis. Force that pushes
molecule through the matrix based on their charge.
Factors affecting rate of migration of the ions Right Ans - Net charge
Size and shape
Buffer pH
Strength of electric field
Properties of medium
Temperature of system
Types of Buffers Right Ans - Barbitone (serum protein)
Phosphate (enzyme)
TBE + TAE (nucleic acids)
Tris-glycine (proteins)
Media Right Ans - Paper
Agar
Cellulose acetate strip
Starch
Agarose
Acrylamide
Types of Electrophoresis Right Ans - Moving boundary/frontal
Zonal (ones we use)
Immuno Electrophoresis
Moving Boundary/ Frontal Electrophoresis Right Ans - Solution injected in
U tube
Arms filled with buffer
, When electricity passed, separate into bands of proteins, no discrete zones.
Zonal Electrophoresis Right Ans - Sample as narrow band
Separation in form of discrete bands
Support media.
Paper Electrophoresis Right Ans - Volume placed along line drawn on
Whatmann paper.
Ends soaked in buffer.
Electricity passed.
Starch Gel Electrophoresis Right Ans - Isoenzymes.
Partially hydrolysed potato starch is used.
Opaque
Non denatured proteins can be separated.
Visualised via staining.
Cellulose Acetate Electrophoresis Right Ans - Strips of cellulose acetate.
Better resolving power
No absorption
No trailing, transparent
Used for plasma proteins.
Gel Electrophoresis Right Ans - Gelatinous material such as agarose,
acrylamide or cellulose acetate.
Support medium to separate samples.
Agarose Gel Electrophoresis Right Ans - Analyse DNA fragments.
Gel from agarose (derivative of agar)
Pores act as a seive and affect the mobility of sample on the medium. Form
bands. No electric charge.
Polyacrylamide Gel Electrophoresis (PAGE) Right Ans - Acrylamide and a
cross linker, methylene bis-acrylamide. Synthetic. Resolves 1bp difference.
Capillary Gel Electrophoresis Right Ans - Thin glass capillary (25 to 100
micrometer diameter)
Polyacrylamide for sieving
Rapid than slab gels
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Zendaya. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $9.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75057 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now