HEMATOLOGY ROTATION WEEK 4
QUESTIONS AND ANSWERS WITH
SOLUTIONS 2024
- ANSWER In the acute AMLs with the WHO classification, how can you distinguish AML with minimal
differentiation from AML without maturation? How can you distinguish that form from AML with
maturation? (Hint: there's a magic number for the granulocytes, and you'll find it in the university lecture
from this material).
- ANSWER Which of the T cell subsets is called "helper cells"? Which are "suppressor cells"? Which
subset is infected in HIV, and decreased in AIDS? What is the threshold number used as diagnosis for
AIDS?
- ANSWER What form of leukemia can follow Burkitt's lymphoma? Do the cells produce TdT?
Basophils - ANSWER CML
-Fibrosis of marrow
-Extramedullary hematopoiesis (spleen/liver)
-Leukoerythroblastosis and teardrops - ANSWER What is the classic triad associated with PMF?
Increased uric acid, LD, and B12 - ANSWER Describe the chemistry results associated with CML.
ETO-AML1 or RUNX1-ETO - ANSWER What is the gene-hybrid name of t(8;21)?
-Normal: 20-100
-CML: <13
-Leukemoid Reaction: >100
-Polycythemia vera: 100-200 - ANSWER List the expected LAP values for Normal, CML, Leukemoid
Reaction, and Polycythemia vera.
, Nonspecific Esterase
(Alpha napthyl acetate and butyrate) - ANSWER Esterase stain that stains monocytes.
Heparin - ANSWER What is the anticoagulant for bone marrow specimens?
- ANSWER When will you see most of the cells positive with MPO and SBB?
a. ALL
b. AML
c. CLL
d. erythroid leukemia
- ANSWER What nuclear enzyme is a marker for primitive lymphocyte populations? What anticoagulant
should not be used when testing for this marker?
- ANSWER Flow Cytometry labels cells using antibodies labeled with what?
- ANSWER What anticoagulant is recommended for whole blood and bone marrow for flow cytometric
testing?
- ANSWER What does Propidium Iodide stain?
- ANSWER Flow Cytometry measures forward scatter and side scatter. What does each indicate about
the cells?
Forward scatter: ___ Side scatter: ___
- ANSWER What procedural step is necessary for fluorescent antibodies to react with cytoplasmic
and/or nuclear antigens?
- ANSWER What does FISH stand for? Give an example of a disease that can be diagnosed by FISH.
QUESTIONS AND ANSWERS WITH
SOLUTIONS 2024
- ANSWER In the acute AMLs with the WHO classification, how can you distinguish AML with minimal
differentiation from AML without maturation? How can you distinguish that form from AML with
maturation? (Hint: there's a magic number for the granulocytes, and you'll find it in the university lecture
from this material).
- ANSWER Which of the T cell subsets is called "helper cells"? Which are "suppressor cells"? Which
subset is infected in HIV, and decreased in AIDS? What is the threshold number used as diagnosis for
AIDS?
- ANSWER What form of leukemia can follow Burkitt's lymphoma? Do the cells produce TdT?
Basophils - ANSWER CML
-Fibrosis of marrow
-Extramedullary hematopoiesis (spleen/liver)
-Leukoerythroblastosis and teardrops - ANSWER What is the classic triad associated with PMF?
Increased uric acid, LD, and B12 - ANSWER Describe the chemistry results associated with CML.
ETO-AML1 or RUNX1-ETO - ANSWER What is the gene-hybrid name of t(8;21)?
-Normal: 20-100
-CML: <13
-Leukemoid Reaction: >100
-Polycythemia vera: 100-200 - ANSWER List the expected LAP values for Normal, CML, Leukemoid
Reaction, and Polycythemia vera.
, Nonspecific Esterase
(Alpha napthyl acetate and butyrate) - ANSWER Esterase stain that stains monocytes.
Heparin - ANSWER What is the anticoagulant for bone marrow specimens?
- ANSWER When will you see most of the cells positive with MPO and SBB?
a. ALL
b. AML
c. CLL
d. erythroid leukemia
- ANSWER What nuclear enzyme is a marker for primitive lymphocyte populations? What anticoagulant
should not be used when testing for this marker?
- ANSWER Flow Cytometry labels cells using antibodies labeled with what?
- ANSWER What anticoagulant is recommended for whole blood and bone marrow for flow cytometric
testing?
- ANSWER What does Propidium Iodide stain?
- ANSWER Flow Cytometry measures forward scatter and side scatter. What does each indicate about
the cells?
Forward scatter: ___ Side scatter: ___
- ANSWER What procedural step is necessary for fluorescent antibodies to react with cytoplasmic
and/or nuclear antigens?
- ANSWER What does FISH stand for? Give an example of a disease that can be diagnosed by FISH.
