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MIP 302 Final Test Questions with All Correct Answers

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MIP 302 Final Test Questions with All Correct Answers What can the API test strip identify? - Answer-Gram negative rods Why is water placed in the bottom of the API test strip? - Answer-Because the media is small it will dry up making tests hard to decipher What does an underlined well me...

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  • September 4, 2024
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MIP 302 Final Test Questions with
All Correct Answers

What can the API test strip identify? - Answer-Gram negative rods

Why is water placed in the bottom of the API test strip? - Answer-Because the media is
small it will dry up making tests hard to decipher

What does an underlined well mean on an API strip? - Answer-Fill tube and add oil

What does a boxed well mean on an API strip? - Answer-Fill Tube and cupule with
bacteria

What does a well that is not underlined or boxed mean? - Answer-Fill just the tube

Where in API codebook should you look if Oxidase (-)? - Answer-In white section

Where in API codebook should you look if Oxidase (+)? - Answer-In blue section

When is bacteria Oxidase (+)? - Answer-If isolate is gram negative rod that does not
ferment glucose

When is bacteria Oxidase (-)? - Answer-If gram negative rod does ferment glucose

What is direct Elisa testing for? - Answer-Particular antigen

What is indirect Elisa testing for? - Answer-Antibody

Positive ELISA result - Answer-Color in well

Negative ELISA result - Answer-Colorless well

Titer - Answer-Dilution x volume used
-Reciprical of that answer

Why is blocking important? - Answer-To ensure clean results so that non specific
binding can not occur which would give a false positive result

3 types of selective media - Answer-PEA agars, MacConkey agar, and Simmons citrate
agar slants

, Component of PEA agar? - Answer-0.25% Phenyl Ethyl Alcohol

antigen capture ELISA - Answer-antibody directed against a target antigen is coated
directly onto a solid matrix. Binds with any antigen present in the sample. Used to detect
presence of a particular antigen but also used to detect presence of antibody directed
against a particular antigen.

antibody capture ELISA - Answer-directed against a particular antibody type, is coated
directly onto a solid matrix. Second antibody becomes sandwiched between the capture
antibody and the antigen. Captured by the antibody bound to the solid matrix.

Indirect elisa - Answer-detect presence of specific antibody directed against a particular
antigen, or presence of a particular antigen, enzyme will only be present to react with
substrate if the secondary antibody that it is attached to which is also attached to the
primary antibody

direct elisa - Answer-detect presence of particular antigen in a sample, antibody
carrying reporter enzyme is bound directly to the target antigen


Purpose of Blood Agar - Answer-See if bacteria has hemolysis abilities

Purpose of Catalase Test - Answer-Determine if bacteria produces catalase

Purpose of Indole Test - Answer-See if bacteria produces tryptophanase

Medium for Indole test - Answer-Tryptone broth

Purpose of Klinglers Iron Agar - Answer-See ability of bacteria to ferment glucose
and/or lactose and the ability to produce CO2 and H2S gases

Purpose of Lysine Decarboxylase Broth - Answer-Determine if bacteria produces Lysine
Decarboxylase

Purpose of MacConkey Agar - Answer-See if bacteria is gram negative and can ferment
lactose

Purpose of Methyl Red test - Answer-See if bacteria ferments glucose and produces
mixed acids

Medium from Methyl Red test - Answer-MRVP broth

Purpose of Nitrate Broth - Answer-Distinguish bacteria based on their ability to reduce
nitrate to nitrite

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