100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
MIP 302 Exam 1 Questions with Answers All Correct $12.49
Add to cart
Quickly navigate to
Preview
  2.  
  3. MIP 302
  4.  
  5. MIP 302

Exam (elaborations)

MIP 302 Exam 1 Questions with Answers All Correct

 0 purchase
  • Course
  • MIP 302
  • Institution
  • MIP 302

MIP 302 Exam 1 Questions with Answers All Correct What does a broth being turbid represent? - Answer-bacterial growth within broth Why would you incubate the TSB inoculated with normal flora at 37 degrees? (37 degrees Celsius is equal to 98.6 Fahrenheit) - Answer-37 degrees Celsius is the temp...

[Show more]

Preview 2 out of 5  pages

Document information

  • September 4, 2024
  • 5
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

Subjects

  • mip 302
  • mip 302 exam 1
  • mip 302 exam 1 questions with answers all correct
  • what does a broth being turbid represent

Written for

  • MIP 302
  • MIP 302

Seller

avatar-seller
Scholarsstudyguide

Reviews received

Content preview

MIP 302 Exam 1 Questions with
Answers All Correct
What does a broth being turbid represent? - Answer-bacterial growth within broth

Why would you incubate the TSB inoculated with normal flora at 37 degrees? (37
degrees Celsius is equal to 98.6 Fahrenheit) - Answer-37 degrees Celsius is the
temperature of human body, therefore this mimics normal conditions for the normal flora

Why do invert a plate before incubating it? - Answer-so no condensation from lid drops
into bacteria in the hot environment of the incubator

Where do you write your name and section number on the plate? why? - Answer-on the
bottom so that the plate can still be identified even if it is separated from the lid

Why is broth not used as an isolation medium? - Answer-the different types of bacteria
that grew cannot be separated or identified

Would you use a TSA slant as an isolation medium? - Answer-no, the slant does not
allow enough surface area for growth or enough access

Why is it important that the TSA slant be inoculated with a pure bacterial culture, instead
of a mixed culture? - Answer-pure bacterial cultures must be used in order to properly
identify the identity of the bacteria in the sample

Gram stain procedure - Answer-1: primary stain - crystal violet (purple)
2: mordant- grams iodine (binds to crystal violet, still purple)
3: decolorizer- alcohol/acetone mixture (removed iodine from gram negative, gram + is
purple, gram - is colorless)
4: counterstain - safranin (turns gram - cells pink)

gram + appear what color - Answer-purple

gram - appear what color - Answer-pink

acid-fast staining procedure - Answer-1: primary stain - carbon fuchsin + heat (all cells
hot pink/red)
2: decolorized- acid alcohol mix (acid-fast + are pink, acid-fast - are colorless)
3: secondary stain - methylene blue (acid-fast - are blue)

endospores stain what color - Answer-green

vegetative cells stain what color - Answer-pink

, acid-fast + appear what color - Answer-pink/red

acid-fast - appear what color - Answer-blue

endospore staining procedure - Answer-1: primary stain - malachite green + heat (all
green at this point)
2: decolorizer - water (washes off excess stain out of vegetative cells, endospores =
green, vegetative cell = colorless)
3: secondary stain - safranin (endospores = green, vegetative cells = pink)

why do gram positive cells stain purple, and gram negative cells stain pink? - Answer-
gram + cells stain purple because they have a thick peptidoglycan layer that is too thick
for the decolorizer to remove the grams iodine, whereas gram - cells have a thin ppg
that allow for the removal of the mordant and for them to stain pink


What should you do if you get a skin cut in the lab? - Answer-Report to instructor

what should you do if you drop a test tube that contains bacterial cultures and it shatters
on the floor? - Answer-report to instructor & dispose of waste in the manner prescribed
by instructor

when should you mouth pipette? - Answer-never

list 3 items that should be discarded in the red incinerator bin - Answer-plastic tubes,
glass slides, swabs, gloves

when should you decontaminate your bench with disinfectant - Answer-when you enter
and complete lab

when is it okay to eat/drink in lab - Answer-never

what is "proper lab attire" - Answer-lab coat, close-toed shoes, safety goggles, long hair
pulled back

how should test tubes be stored when you are using them at your bench? - Answer-in
cups or racks

what is the guest policy - Answer-guests are not allowed

what criteria did you use to determine if a cell is alga or protozoan - Answer-algae: not
moving, yellow/green color
Protozoa: darker colors, moving

why are many algae green or yellow in color - Answer-because they are photosynthetic

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller Scholarsstudyguide. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $12.49. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

69252 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 15 years now

Start selling
$12.49
  • (0)
Add to cart
Added