OSMT Histology Exam Questions and Answers All Correct
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Course
OSMT Histology
Institution
OSMT Histology
OSMT Histology Exam Questions and Answers All Correct
What are 3 transport medias they use for tissues - Answer-1) Saline- for unfixed STAT
2) Michaels transport medium
3) fixative
What is michaels transport medium - Answer-it is commercial saline-like preservatives for unfixed tissue to ...
OSMT Histology Exam Questions
and Answers All Correct
What are 3 transport medias they use for tissues - Answer-1) Saline- for unfixed STAT
2) Michaels transport medium
3) fixative
What is michaels transport medium - Answer-it is commercial saline-like preservatives
for unfixed tissue to allow for transportation or delay of fixation
What is the best transport medium to use for tissues - Answer-fixative
When transporting wet tissue specimens what type of packing is it and what is on the
label - Answer--in a plastic leak proof container with screw cap lids
-patient info
-whmis labels
-clearly labelled with shipping and return address
When transporting slides what packaging are they sent in - Answer-cardboard or plastic
mailing envelopes
What are specimens called in order to remove it , it has to be done by surgery - Answer-
irretrievable specimen
What is grossing - Answer-looking at the sample in greater detail
-examining and describing
What are block sections - Answer-sections that are cut from the tissue that are being
examined for interest
What is the thickness of an ideal block section - Answer-3-4 mm thick
Where are block sections placed into - Answer-cassettes
What are some specimens (objects) that are not processed - Answer-bullets, implants,
impaled objects
What is fixation - Answer-to examine tissue thoroughly to harden and preserve
What is the aim of fixation - Answer-to preserve the tissue in a lifelike manner
, What the key of fixation - Answer-stabilize proteins
What is the goal of fixation - Answer-to prevent putrefaction which is the destruction of
cells by bacterial pathogens or normal flora
what are the effects of fixation (4) - Answer-1) hardens tissue
2) makes tissue easier to work with
3) protects tissue from hard chemicals
4) enhances staining by making the tissue more receptive to dyes
what happens if the tissue over hardens - Answer-the tissue shrinks and becomes brittle
what factor influence fixation - Answer-higher temps= faster fixation
what is the ideal temp for fixation - Answer-usually done at RT (22)
how can agitation help with fixation - Answer-leads to faster and better fixation
leads to better penetration of fixative
how much fixative should you have ? - Answer-20x the volume of tissue
what happens if the tissue is to dense - Answer-longer to fix
Are CT capsules difficult to penetrate - Answer-yes
how do you fix hollow organs - Answer-fill or pin flat
what size should an ideal tissue block be - Answer-the size of a nickel
what are the 3 kinds of fixative categories - Answer-physical , simple and compound
what are physical fixatives - Answer-heat, microwave, freeze dry
what are simple fixatives - Answer-use once chemical , called primary fixative
what are compound fixatives - Answer-2 or more fixatives
-combination of one or more
what is the workflow for histology (7) - Answer-1) specimen collection
2) grossing
3) fixation
4) processing
5) embedding
6) microtomy
7) routine staining
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