100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
MCB 2610 Lab Exam I Questions And Answers Rated 100% Correct $7.99
Add to cart
Quickly navigate to
Preview
  2.  
  3. MCB 2610 Lab
  4.  
  5. MCB 2610 Lab

Exam (elaborations)

MCB 2610 Lab Exam I Questions And Answers Rated 100% Correct
 0 purchase
  • Course
  • MCB 2610 Lab
  • Institution
  • MCB 2610 Lab

why is the spore stain used with bacteria such as Bacillus and Clostridium? - ️️they develop an endospore in response to certain environmental conditions such as depletion of nutrients procedure for making smears - ️️1. use a wax pencil to draw a circle on the slide 2. take a loop of di...

[Show more]

Preview 2 out of 8  pages

Document information

  • October 14, 2024
  • 8
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

Subjects

Written for

  • MCB 2610 Lab
  • MCB 2610 Lab

Seller

avatar-seller
ACADEMICMATERIALS

Reviews received

Content preview

MCB 2610 Lab Exam I
why is the spore stain used with bacteria such as Bacillus and Clostridium? - ✔️✔️they
develop an endospore in response to certain environmental conditions such as
depletion of nutrients

procedure for making smears - ✔️✔️1. use a wax pencil to draw a circle on the slide
2. take a loop of distilled water and smear it on the slide
3. take a very small loop of culture
4. smear it onto distilled water
5. allow to dry then heat fix, smear side up 3x

what is the purpose of aseptic technique? - ✔️✔️maintain pure cultures

what is the purpose of a T-streak? - ✔️✔️isolate and maintain single species from
mixed populations

which objective lens should never be used with immersion oil? - ✔️✔️40x


what type of stain is used for simple staining? - ✔️✔️simple crystal violet stain

if the chromophore is a positive ion, the stain is considered a(n)
______________________ stain - ✔️✔️basic

if the chromophore is a negative ion, the stain is considered a(n) __________________
stain - ✔️✔️acidic

what is the quickest and most economical method of bacterial isolation? - ✔️✔️streak
plate method

what is the mordant used in the gram stain and what does it do? - ✔️✔️iodine - it
makes the color permanent

bacteria that retain the Gram stain are classified as Gram _____________ -
✔️✔️positive

what do Gram positive bacteria have that allows them to retain the primary stain? -
✔️✔️thick wall of peptidoglycan

why are Gram negative bacteria easy to decolorize? - ✔️✔️they have an outer
membrane and a thinner layer of peptidoglycan

, do Gram negative stains retain the counterstain (safranin)? - ✔️✔️yes


T/F heat used during staining causes sporulation - ✔️✔️FALSE

Procedure for Gram Stain - ✔️✔️1. prepare a smear of bacteria
2. flood the surface with crystal violet
3. wait one minute and then pour off the excess stain
4. wash gently with water
5. flood the stain with Gram's iodine
6. wait one minute and then wash off the excess with water
7. drip alcohol on the slide and allow to decolorize until no more color is removed
8. wash with water
9. counterstain for 30-40 seconds with safranin
10. wash with water
11. blot dry

microbial growth is defined as
_________________________________________________________ - ✔️✔️an
increase in population size

what is happening during the lag phase? - ✔️✔️cells are adjusting to new conditions
and synthesizing a full complement of enzymes

what is happening during the log phase? - ✔️✔️each cell divides into two new cells -
two-fold exponential growth


what is happening during the death phase? - ✔️✔️cell death rate exceeds remaining
cell division

what causes the death phase? - ✔️✔️accumulation of toxic waste, cell damage from
environment

as bacteria grow in a liquid medium, the broth becomes ___________ turbid, which
_____________ optical density - ✔️✔️more, increases

how can we measure the change in optical density? - ✔️✔️spectrophotometer

the change in optical density is AKA __________________ - ✔️✔️absorbance

what is the generation time? - ✔️✔️the time required for the population to double in
number

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller ACADEMICMATERIALS. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $7.99. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

69052 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 15 years now

Start selling

Recently viewed by you

Exam (elaborations) ·

(0)

Cpls course test 2 with complete solutions
$7.99
  • (0)
Add to cart
Added