translatio al Protein Processing
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1. overview of translation: only by the
composition
In the process of translation, the uses an mRNA of the
template and aminoacyl- to synthesize a . also the
Messenger RNA is read 5' 3' and the polypeptide
is synthesized from the - to the -
terminus.
amino acids are attached to the end
2. Activation of amino acids for incorporation into
pro- teins by attachment to a
The anticodon loop contains a trinucleotide
sequence called the that is to the
appropriate trinu- cleotide codon in the .
3. Synthetic nucleotides were used to decipher the
ge- netic code
In experiments with and nucleotides, only
pro- line and arginine were produced, respectively
The polymer UCUCUCUC...
produced Ser-Leu-Ser-Leu...
However, the AAGAAGAAG... polymer produced
polylysine, polyarginine and polyglutamate
(above)
The nature of the peptide product is determined not
, BMS 528: CH. 25- Informational Decoding: Translation and Post-
translatio al Protein Processing
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ribosome tRNAs polypeptide
NC
3'
tRNA
anticodon complementary mRNA
polyC poly A
mRNA
reading frame first
, BMS 528: CH. 25- Informational Decoding: Translation and Post-
translatio al Protein Processing
Study online at https://quizlet.com/_gc77bj
(the trinucleotide selected for the amino acid
incorporation)
4. the genetic code: AUG
methionine
The start codon encodes N-formylmethionine if
used as a start codon in prokaryotes ( if used TRUE: UUG,
as a start codon in eukaryotes) AUU, GUG
T/F: there are Rare bacterial start codons other nonsense
than AUG. amino acid
UAA
The three stop codons, also called codons UAG
because they do not code for an :
-
- UGA amino acids
- stop codons
translation
matching
Sense codons- code for - anti- codon
Nonsense codons- are . ends. There are
no aminoacyl tRNAs with
5. modifications to the genetic code: genetic code
Some organelles and organisms deviate from the mitochondrial
standard encoding the 20 standard amino stop codons
acids
Of note are selenocysteine and pyrrolysine (the 21st
and 22nd amino acids)
DNA has slightly different codons 21st
and 22nd. Sec and Pyl