BCH5413 Exam 2: RNA Processing
UPDATED ACTUAL Exam Questions and
CORRECT Answers
RNA processing includes - CORRECT ANSWER - capping, polyadenylation, and splicing
of pre-mRNAs to produce mature mRNAs in the nucleus
mRNA is translated by ____ - CORRECT ANSWER - ribosomes
Which end of the DNA is capped? - CORRECT ANSWER - 5'
T or F: pre-mRNA is not capped - CORRECT ANSWER -F
T or F: improperly processed mRNA is rapidly degraded in the nucleus - CORRECT
ANSWER -T
What is mRNA in the nucleus coated with? - CORRECT ANSWER - nuclear proteins
What is mRNA in the cytoplasm coated with? - CORRECT ANSWER - cytoplasmic
proteins
Ribonucleoprotein (RNP) complexes - CORRECT ANSWER - consist of mRNAs,
ncRNAs, and proteins
e.g. spliceosome, RISC, ribosome
Role of spliceosome, RISC, ribosome - CORRECT ANSWER - pre-mRNA splicing,
mRNA silencing, translation
, Role of the 5' cap in the nucleus - CORRECT ANSWER - 1. efficient pre-mRNA
processing and export
2. protect pre-mRNA from degradation by 5' exonucleases
3. 3' processing of pre-mRNA (cleavage and polyadenylation)
4. RNA splicing - 5' cap binds to the nuclear cap-binding complex (CBC)
5. mRNA export from the nucleus
Transcripts are capped by ____ nucleotides long - CORRECT ANSWER - 25
T or F: all the enzymes required for the capping bind to the long CTD of pol II during
transcription initiation - CORRECT ANSWER -T
T or F: the CTD domain serves distinct functions during splicing and polyadenylation -
CORRECT ANSWER -F
T or F: miRNA can control the amount of protein synthesis with a singular mRNA - CORRECT
ANSWER -T
T or F: mRNA stability controls amount of protein synthesis - CORRECT ANSWER -T
T or F: level of phosphorylation of the CTD domain impacts the binding proteins - CORRECT
ANSWER -T
T or F: Pol I and Pol III do not have long CTD and do not have capping abilities - CORRECT
ANSWER -T
T or F: miRNAs are made by Pol II (i.e. are they capped?) - CORRECT ANSWER -T
How is the 5' cap linked to the mRNA structurally - CORRECT ANSWER - 5' to 5'
linkage
UPDATED ACTUAL Exam Questions and
CORRECT Answers
RNA processing includes - CORRECT ANSWER - capping, polyadenylation, and splicing
of pre-mRNAs to produce mature mRNAs in the nucleus
mRNA is translated by ____ - CORRECT ANSWER - ribosomes
Which end of the DNA is capped? - CORRECT ANSWER - 5'
T or F: pre-mRNA is not capped - CORRECT ANSWER -F
T or F: improperly processed mRNA is rapidly degraded in the nucleus - CORRECT
ANSWER -T
What is mRNA in the nucleus coated with? - CORRECT ANSWER - nuclear proteins
What is mRNA in the cytoplasm coated with? - CORRECT ANSWER - cytoplasmic
proteins
Ribonucleoprotein (RNP) complexes - CORRECT ANSWER - consist of mRNAs,
ncRNAs, and proteins
e.g. spliceosome, RISC, ribosome
Role of spliceosome, RISC, ribosome - CORRECT ANSWER - pre-mRNA splicing,
mRNA silencing, translation
, Role of the 5' cap in the nucleus - CORRECT ANSWER - 1. efficient pre-mRNA
processing and export
2. protect pre-mRNA from degradation by 5' exonucleases
3. 3' processing of pre-mRNA (cleavage and polyadenylation)
4. RNA splicing - 5' cap binds to the nuclear cap-binding complex (CBC)
5. mRNA export from the nucleus
Transcripts are capped by ____ nucleotides long - CORRECT ANSWER - 25
T or F: all the enzymes required for the capping bind to the long CTD of pol II during
transcription initiation - CORRECT ANSWER -T
T or F: the CTD domain serves distinct functions during splicing and polyadenylation -
CORRECT ANSWER -F
T or F: miRNA can control the amount of protein synthesis with a singular mRNA - CORRECT
ANSWER -T
T or F: mRNA stability controls amount of protein synthesis - CORRECT ANSWER -T
T or F: level of phosphorylation of the CTD domain impacts the binding proteins - CORRECT
ANSWER -T
T or F: Pol I and Pol III do not have long CTD and do not have capping abilities - CORRECT
ANSWER -T
T or F: miRNAs are made by Pol II (i.e. are they capped?) - CORRECT ANSWER -T
How is the 5' cap linked to the mRNA structurally - CORRECT ANSWER - 5' to 5'
linkage
