Bcmb 406b - Study guides, Class notes & Summaries
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BCMB 406B Midterm 1 Test Latest Update
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BCMB 406b Test 1-Questions with 100% Correct Verified answers
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what two criteria need to be meet when picking a replacement residue in SDM - Answer 
remove charge of functional group of original residue 
be similar or smaller in size 
What are the most significant problems in primer design - Answer secondary structures 
(loses primer), 3' end stability (mispriming), Tm difference (hard to set up) 
Primer length - Answer 18-22bp
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BCMB 406B Midterm 1 lab 1 exam with complete solutions
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PCR allows for: 
amplification of minute amounts of template DNA 
can generate thousands or millions of copies of that sequence 
 
 
 
What is one of the most important parameters for a successful PCR? 
well designed primers 
 
 
 
 
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Name 3 variables that influence the success of a PCR reaction 
1. quality of template DNA 
2. cycling conditions 
3. specificity and stabilit...
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BCMB 406B Midterm 1 Lab 1 Test Latest Update
- Exam (elaborations) • 19 pages • 2024
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BCMB 406b Test 1 with answers
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what variables are considered in primer design - length, sequence, %GC, Tm, Ta, 3'end stability, 5' end stability, secondary structures 
 
why is the formation of secondary structures in primers especially undesirable - reduces primer available to use to make product 
 
standard denaturation temp and time (initial and denaturation) - 94C 
1-5min initial 
20-45 sec 
 
std annealing temp and time - temp varies between protocols, 30s 
 
std elongation temp and time - temp depends on polymerase u...
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BCMB 406B - Lab exam questions and solutions bundle!!
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BCMB 406B - Lab exam questions and solutions bundle!!
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BCMB 406B - Lab 1 Test With Complete Solution
- Exam (elaborations) • 10 pages • 2024
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BCMB 406B - Lab 2 Electroporation Controls Test-Questions with 100% Correct Verified answers
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0.1 ng vs. 1 ng pET28a w/ Kan selection - Answer - Showed that the amount of pET28a 
plasmid DNA doesn't affect the transformation efficiency 
0.1 ng vs 0.1 ng (1/10 dilution) pUC19 w/ Kan selection - Answer - Showed that the 
amount of pUC19 present influenced the transformation efficiency 
Untreated CBM26-3 PCR product - Answer - Shows that the WT DNA can be transformed 
and grow on a plate without the DpnI treatment
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BCMB 406B - Lab 2 Test Study Guide
- Exam (elaborations) • 6 pages • 2024
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BCMB 406B Lab Questions and Answers
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BCMB 406B Lab Questions and Answers
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