Inoculum - Study guides, Class notes & Summaries
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BIOS 242 Week 1 Assignment; Lab 2 of 14 Onsite - Isolation of Pure Cultures
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BIOS 242 Week 1 Assignment; Lab 2 of 14 Onsite - Isolation of Pure Cultures 
 
Lab 2: Techniques for Isolation of Pure Cultures 
 
 
Learning Objectives: 
 
 
Isolate pure cultures from a mixed population of microorganisms using aseptic technique. 
Learn the streak plate method 
 
 
 
 
Introduction: 
 
 
Microorganisms exist in nature as mixed populations of different species. In order to study them and observe their characteristics it is first necessary to obtain pure cultures (a culture ...
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BIOL 2460 Chapter 9: Microbial Growth Graded A+
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BIOL 2460 Chapter 9: Microbial Growth 
 
Graded A+ 
 
Most common form of bacterial reproduction 
Binary fission 
4 Basic Steps of Binary Fission 
1. Growth of cell size and increase in cell components 
2. Replication of DNA/chromosomes 
3. Division of cytoplasm (cytokinesis) 
4. Septum formation and division of daughter cells 
 
Cytokinesis is directed by the ___________ protein. 
 
FtsZ 
 
Where is the Z ring assembled during binary fission? 
On the cytoplasmic membrane 
Divisome activates the...
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BIOL 2420 Lab Practical 1 Test Questions and Correct Answers
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What is the single most important way to reduce the spread of unwanted microbes? handwashing 
Where and how should used media (culture tubes and plates) be disposed of? directly into bio hazard bin 
Where and how should a slide prepared by you (ex: Gram stain) be disposed of? Wrapped in a paper towel and disposed into the biohazard bin 
Where should gloves worn by you (after lab, not cleaning up a spill) be disposed of? in the regular trash can 
Where should a broken stock slide (ex: 3 forms sli...
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UTA Micro 2460 Chapter 9 Correctly Answered Questions| UpToDate | Already Graded A+
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Binary Fission 
: Most common form of bacterial reproduction 
Binary Fission 
4Basic Steps 
: 1.Growth of cell size and increase in cell components 
2.Replication of DNA 
3.Division of the cytoplasm (cytokinesis) 
4.Septum formation and division of daughter cells 
Z ring Assembly 
: •Cytokinesis is directed by FtsZ protein 
•FtsZ assembles Z ring to form divisome 
•Divisome activates production of peptidoglycan and septum 
Generation Time 
: •Generation Time (Doubling Time) - time takes ...
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BIOS 242 Lab 1: Culture Transfer Techniques
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Lab 2: Techniques for Isolation of Pure Cultures 
Learning Objectives: 
 Isolate pure cultures from a mixed population of microorganisms using aseptic technique. 
 Learn the streak plate method 
VIDEO: 
 Making a streak Plate (Bio-Rad Laboratories) 

Introduction: 
Microorganisms exist in nature as mixed populations of different species. In order to study them and 
observe their characteristics it is first necessary to obtain pure cultures (a culture containing only one 
species of an o...
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Nursing Microbiology Chapter 9 (2460 UTA) OpenStax Book Questions and Correct Answers
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Binary Fission Most common form of bacterial reproduction 
Binary fission 4 Basic steps 1. Growth of cell size and increase in cell components 2. Replication of DNA 3. Division of Cytoplasm (cytokinesis) 4. Septum formation and division of daughter cells 
Fstz 1. Cytokinesis is directed by this protein 2. Assembles Z ring to form (Divisome) 
What activates the production of a peptidoglycan and septum? Divisome 
Generation (Doubling Time) Time takes to double population through one round of binar...
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UTA Microbiology Exam 3 Correctly Answered Questions| UpToDate | Already Graded A+
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4 Basic Steps of binary fission 
: 1. Growth of cell size 
2. Replication of DNA 
3. Division of cytoplasm 
4. Septum formation & cell division 
Z Ring Assembly 
: FtsZ assembles Z ring to form divisome 
Generation Time 
: time it takes to double the population 
Lag Phase 
: Cells adjust to culture medium; no change in population 
What factors determine the duration of the lag phase? 
: Genetic makeup, media composition, initial inoculum size 
During which phase of the growth curve is it most ap...
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Community Acquired Pneumonia (CAP) Questions and Answers Already Passed
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CAP is the ___________ infectious ds in the US and the ______________ leading cause of 
death. - Most deadly 
- 8th 
What normally prevents the development of lower respiratory tract infections following 
aspiration of oropharyngeal secretions containing bacteria or inhalation of infected aerosols? 
Pulmonary defense mechanisms (cough reflex, mucociliary clearance system, immune 
responses) 
CAP occurs when _________________ . There is a defect in 1 or more of the normal 
defense mechanisms (cou...
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UTA Microbiology Exam 3 Questions With 100% Correct!!
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4 Basic Steps of binary fission - Answer-1. Growth of cell size 
2. Replication of DNA 
3. Division of cytoplasm 
4. Septum formation & cell division 
Z Ring Assembly - Answer-FtsZ assembles Z ring to form divisome 
Generation Time - Answer-time it takes to double the population 
Lag Phase - Answer-Cells adjust to culture medium; no change in population 
What factors determine the duration of the lag phase? - Answer-Genetic makeup, media composition, 
initial inoculum size 
During which phase of...
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ASCP BOC Practice Exam 2023 Correct 100%
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CML vs. Leukemoid Reaction - ANSWER CML - decreased leukocyte alkaline phosphatase (LAP) 
Leukemoid Reaction - increased LAP 
 
Look-back - ANSWER The process for the identification and notification of recipients of blood components from prior donations of who now test positive for HCV, HIV-1 and/or HIV-2. 
 
External reference electrode used in ISE - ANSWER KCl 
 
The serum glucose concentration of a diabetic patient undergoing a 2-hr GTT should return to the baseline (fasting level) after ____...
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