Mutagenesis - Study guides, Class notes & Summaries

Lecture 22 Protein Engineering Exam Questions With Verified Answers. What is protein engineering? - answermodification of a protein with recombinant DNA technology to get a better use in medicine Enzymes work more efficiently because they are __________ in harsh conditions. - answerstable General Methods for Gene Mutation: Method 1 - Classic Mutagenesis by Radiation: UV lights cross-link two neighboring ________________ bases in DNA. Errors and mutations are introduced during DNA replica...

MMSC490 Exam 2 Questions with Complete Answers Which eukaryotic DNA polymerase replicates the lagging strand in the 3ʹ to 5ʹ direction? a. α b. γ c. δ d. None of the above; the lagging strand is replicated in the 5ʹ to 3ʹ direction. - Answer ️️ -d. None of the above; the lagging strand is replicated in the 5ʹ to 3ʹ direction. DNA polymerases can synthesize DNA: a. de novo, by catalyzing the polymerization of free dNTPs. b. by adding dNTPs to complementary dNTPs on a single-...

Forward genetic analysis starts with... CORRECT ANSWER ...a genetic screen that identifies specific phenotypic abnormalities in a population of organisms that have been mutagenized-mutagenesis being the intentional introduction of mutation into the genome of an organism Reverse genetics approaches begin... CORRECT ANSWER ...with a gene sequence and seek to identify the corresponding mutant phenotype Saturation mutagenesis CORRECT ANSWER Mutagenesis aimed at identifying multiple mutant alle...

How does CRISPR work naturally? Correct Answer Clustered Regularly Interspaced Short Palindromic Repeats - bacterial defense mechanism - bacteria have short palindromic repeats separated by spacer regions which are segments of DNA from past viral exposure - when attacked again by same virus, the spacer region is used as a template and the guide RNA (tracrRNA) helps recognize the viral DNA and degrade it using Cas9 nuclease at the specific site - need PAM (helps distinguish between self and n...

MMSC 490 Exam 2 Questions with Correct Answers Which eukaryotic DNA polymerase replicates the lagging strand in the 3ʹ to 5ʹ direction? a. α b. γ c. δ d. None of the above; the lagging strand is replicated in the 5ʹ to 3ʹ direction. - Answerd. None of the above; the lagging strand is replicated in the 5ʹ to 3ʹ direction. DNA polymerases can synthesize DNA: a. de novo, by catalyzing the polymerization of free dNTPs. b. by adding dNTPs to complementary dNTPs on a single-strand...

_______ greatly activates the classical pathway of complement activation - IgM _______ is expressed by T cells whose function is to activate other cells. - CD4 ___________ is expressed by cytotoxic T cells - CD8 ____________ molecules present peptides from pathogens, commonly viruses, to CD8 T cells, which are specialized to kill any cell that they specifically recognize. - MHC class I _____________ increases the expression of both MHC Class I and MHC Class II molecules, and can induce the...

RADT 1200 chap 10 Test Questions with Correct Answers What supports the premise that "each pt should get the right imaging examination, at the right time, and with the right radiation dose" - Answer-FDA white paper What is a genetic (hereditary) effect - Answer-Mutagenesis (leads to abnormalities in new cells) What is the current method for controlling the risk of biological damage to radiation workers and the general public from radiation exposure - Answer-EfD Is the upper boun...

What is genetic engineering? - using in vitro techniques to alter genetic material in the lab What is recombinant DNA technology? - the artificial recombination of DNA from two organisms What are some basic techniques of genetic engineering? - DNA amplification electrophoesis nucleic acid hybridization molecular cloning expressing foreign genes targeted mutagenesis What is heterologous expression? - expressing a gene in a different host What is the polymerase chain reaction? - rapid a...

Protein Engineering Exam Questions With Verified Answers. Definition of protein engineering? - answermodification of a protein with recombinant DNA technology to get a better use in medicine, industry, agriculture, and others 2 purposes of protein engineering? - answer1. make enzymes more stable in harsh conditions 2. make enzymes work more efficiently 3 steps of protein engineering? - answer1. modify DNA sequence 2. changed protein sequence 3. improved stability and function First gene...

PCR allows for: amplification of minute amounts of template DNA can generate thousands or millions of copies of that sequence What is one of the most important parameters for a successful PCR? well designed primers Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More Name 3 variables that influence the success of a PCR reaction 1. quality of template DNA 2. cycling conditions 3. specificity and stabilit...