Methylation - Study guides, Class notes & Summaries

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - Answer- ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - Answer- the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - Answer- Hybridizes filter-bound DNA...

ASCP MB: Extra-Practice Questions & Answers 2024 The Klenow fragment of E. coli DNA polymerase I is used: - ANSWER-to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - ANSWER-gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucle...

1. which of the following statements about chickens is false? a- all chickens perceive danger equally b-genetics pre-determines how a chicken responds to danger c-a stress resilient chicken is able to recover from danger and remain healthy d-all of the above ANS: a 2. which of the following could contribute to variation in the stress response of lambs at weaning? a-methylation of the glucocorticoid receptor gene b-polymorphisms in the melancortin 2 receptor gene c-polymorphisms in the nic...

Angelman Syndrome causes (expression and % of total) correct answers **paternal imprinting defect, should be maternal expression 5-7 kb deletion on maternal chr15q11.2-13 (60-70%) UBE3A maternal deletion (11%) Paternal UPD15 (3-7%) Angelman testing strategy and condition features correct answers methylation first since finds ~80% of cases then UBE3A seq and del/dup features: happy demeanor, abnormal gait, seizures, delayed/absent speech Prader-Willi Syndrome causes (expression and...

Genetics Exam #2 With Questions and Answers 100% Solved What is the molecular mechanism for imprinting a gene? a. acetylation b. methylation c. phosphorylation d. mutation e. nitration - answerB Genomic imprinting most commonly uses____ to ____genes. Select one: a. acetylation : activate b. methylation : activate c. phosphorylation : activate d. methylation : deactivate e. phosphorylation : deactivate - answerD Individuals with three copies of most autosomes do not survive. Indivi...

ABGC Boards Overview Questions and Answers 100% Solved Angelman Syndrome causes (expression and % of total) ️️**paternal imprinting defect, should be maternal expression 5-7 kb deletion on maternal chr15q11.2-13 (60-70%) UBE3A maternal deletion (11%) Paternal UPD15 (3-7%) Angelman testing strategy and condition features ️️methylation first since finds ~80% of cases then UBE3A seq and del/dup features: happy demeanor, abnormal gait, seizures, delayed/absent speech Prader-Wil...

When water-soluble vitamins are consumed in excess of body needs, generally the excess is: a. excreted in the feces. b. stored in liver, bone, and adipose tissue. c. excreted in the urine. d. not absorbed. correct answers C A major function of B vitamins is to serve as: a. antibodies. b. a source of energy. c. a part of a coenzyme. d. electrolytes. correct answers C Most animals synthesize their own ascorbic acid from _____. a. glucose b. glutamine c. glycogen d. alanine correc...

Pyrimidine dimers formed in DNA due to UV radiation can be repaired by? SOS repair Double strand break repair Mismatch repair Photoreactivation repair (nucleotide excision repair) Base excision repair - CORRECT ANSWER Photoreactivation repair (nucleotide excision repair) Which of the following repair mechanisms uses methyl groups to distinguish between parental and daughter strands? Answers: Mismatch repair Photoreactivation repair (Nucleotide excision repair) SOS repair Doub...

B form DNA correct answersRight handed, 10.5 bp Lysine (K) and Arginine (R) correct answersAssociated with Histones Bromophenol blue correct answersTracking dye Capillary electrophoresis correct answersMust be ss PFGE buffer correct answersTBE Store RNA > 7 months correct answers-70 ethanol Branched DNA correct answersDNA/RNA targets Methylation or FISH correct answersPWS/AG Nested PCR correct answers2nd step, more specific Denaturation HPLC correct answersDetecting...

ABGC Boards - Final Review Questions and Answers 100% Solved Hereditary Breast and Ovarian Cancer ️️BRCA 1/2 Breast 50-87% Second primary tumor 50-64% BRCA 1: 20-45% BRCA 2: 10-30% BRCA 2: Male breast 6-10% Li-Fraumeni Syndrome ️️-Sarcoma, breast, leukemia, adrenal and many others -May present in almost every way (ie all family members with same cancers or all with different) -Adrenocortical carcinoma considered pathognomonic -50% have some type by 30, 90% by age 60 -TP53 ...