Pcr products - Study guides, Class notes & Summaries

BME Exam 2 Questions and Answers | Latest Version | 2024/2025 | Already Passed 45. **(A) What is Recombinant DNA? (B) What can be produced through this process?** **Answer:** **A) Recombinant DNA consists of DNA molecules created by combining genetic material from different sources.** **B) The products of this process can be the recombinant DNA itself, or the RNA and proteins synthesized from it.** 46. **Explain the process of cloning DNA using bacteria, including (1) the de...

ASCP Exam practice Toolkit Questions with A+ rated answers 2023/2024 E. coli RNA Polymerase II - correct answer DNA template, product: mRNA RNA Polymerase I - correct answer DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II - correct answer DNA template Location: Nucleus Products: mRNA, snRNA RNA Polymerase III - correct answer DNA template Location: Nucleus Product: tRNA, 5s rRNA, snRNA Mitochondrial RNA Polymerase - correct answer DNA temp...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer-(50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer-1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provide ...

The correct answer for this question is 1300 mg/dL. The laboratorian per- formed a 1:4 dilution by adding 0.25 mL (or 250 microliters) of patient sample to 750 microliters of diluent. This creates a total volume of 1000 microliters. So, the patient sample is 250 microliters of the 1000 microliter mixed sample, or a ratio of 1:4. Therefore, the result given by the chemistry analyzer must be multiplied by a dilution factor of 4. 325 mg/dL x 4 = 1300 mg/dL.: After experiencing extreme fatigue and ...

PCR allows for: amplification of minute amounts of template DNA can generate thousands or millions of copies of that sequence What is one of the most important parameters for a successful PCR? well designed primers Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More Name 3 variables that influence the success of a PCR reaction 1. quality of template DNA 2. cycling conditions 3. specificity and stabilit...

MB ASCP CONNECT QUESTIONS AND ANSWERS GRADED A What is translocation is associated with Burkitt's Lymphoma? a. t(18; 14) b. t(9; 22) c. t(8; 14) d. t(15; 17) - Answer ️️ -t(8; 14) TIP: Burkkitt's 8 letters, Locus PF Child Paternity Index LOC-A1 3 2/3 2.18 LOC-B2 7/5 5 0.798 LOC-C3 17/17 9/17 5.21 LOC-D4 12 12 1.37 Based on the data presented in the table above and with a prior probability (PP) of 0.5, the probability of paternity in this case is : a. 92.5% b. 99.5% c. 90.5...

B; The correct answer for this question is 1300 mg/dL. The laboratorian performed a 1:4 dilution by adding 0.25 mL (or 250 microliters) of patient sample to 750 microliters of diluent. This creates a total volume of 1000 microliters. So, the patient sample is 250 microliters of the 1000 microliter mixed sample, or a ratio of 1:4. Therefore, the result given by the chemistry analyzer must be multiplied by a dilution factor of 4. 325 mg/dL x 4 = 1300 mg/dL. - After experiencing extreme fatigue an...

d (primers) - Answer *The specificity of the PCR reaction is determined by which of the following components?* a. Mg2+ concentration b. Deoxynucleotides c. DNA polymerase d. Primers a (primer dimers) - Answer *Complementarity of primer pairs at the 3' ends will result in what artifacts?* a. Primer dimers b. Large mis-primed products c. No amplification d. 5' end degradation of the primers d (secondary structure) - Answer *A DNA sequence with high GC content is more likel...

MCB3020 Exam 3 Bacusmo Questions And Answers 100% Correct Biotechnology use of organisms to form useful products Genetic engineering the deliberate modification of the organisms genetic information by directly changing the sequence of nucleic acids in its genome Recombinant DNA technology procedures used to carry out genetic engineering Cloning generation of a large number of identical DNA molecules 5 basic steps of cloning 1. isolate DNA to be cloned 2. Use restriction enzyme or PCR t...

Which level of protein structure is disrupted through the hydrolysis of peptide bonds? Quaternary Tertiary Primary Secondary - ANSWER Primary The primary structure of a protein is the sequence of amino acids held together by peptide bonds. Peptide bonds are formed by dehydration reactions and disrupted by hydrolysis. A mutation in the beta-hemoglobin gene, which results in the replacement of the amino acid glutamate in position 6 with the amino acid valine, leads to the develop...