Molecular Diagnostics Final questions with correct answers

d (primers) - Answer *The specificity of the PCR reaction is determined by which of the following components?* a. Mg2+ concentration b. Deoxynucleotides c. DNA polymerase d. Primers a (primer dimers) - Answer *Complementarity of primer pairs at the 3' ends will result in what artifacts?* a. Primer dimers b. Large mis-primed products c. No amplification d. 5' end degradation of the primers d (secondary structure) - Answer *A DNA sequence with high GC content is more likely to have what property that interferes with PCR amplification?* a. Tendency to degrade b. Enzyme inactivation c. Lower Tm d. Secondary structure b (nested PCR offers increased specificity and yield of product) - Answer *What is the advantage of a nested PCR procedure?* a. Nested PCR is less labor-intensive. b. Nested PCR offers increased specificity and yield of product