Histotechnology fixation - Study guides, Class notes & Summaries

Histotechnology - Fixatives, StainsAdditive fixatives - correct answers Chemically link, add themselves on to tissue and change it - Mercuric chloride - Chromium tetroxide - Zinc sulfate Nonadoptive fixatives - correct answers Organic compounds that act on tissue without chemically combining with it - Acetone - Alcohols Dissociate bound water molecules from tissue protein groups - Shrinkage, hardening with overexposure Coagulant fixatives - correct answers Establishes a network in...

BOC Histotechnology- Processing Question and answers 2023/2024 verified to pass During microtome, it is noted that many of the tissues are very hard and shrunken. Of the following, the most likely explanation for this problem is that the: - correct answers INFILTRATING PARAFFIN IS TOO HOT. When tissue is processed in paraffin wax that is more than 2 degrees Celsius to 4 degrees Celsius above the melting point, the tissue becomes hard and shrunken. Decalcification of small specimens can be...

Histotechnology Lesson 5.1 and 5.2Fixation (histology) the process of fixing tissues with chemical of physical fixatives to preserve tissues Fixative actions (5) •Preserves the tissues in a life-like condition •Stops abnormal enzyme activity and metabolism •Stops autolysis by inactivating the lysosomal enzymes •Stops bacterial and fungal growth •Stops putrefactive changes Give 2 qualities of a perfect fixative •Works quickly with minimum distortion, penetrate the tis...

Histotechnology A Self-Instructional Text, 4th ed.. Question and answers already passed 2023/2024 For adequate fixation, the fixative volume should be at least ______ times greater than the tissue volume. - correct answers 15-20 This type of microscope is used for the identification of crystals as well as to make identification of amyloid. - correct answers Polarizing microscope For most routine H & E staining, the sections should be 1 cell layer thick, and should be cut at ______ micromet...

An example of an additive fixative is one that contains: CORRECT ANSWER-picric acid Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following: CORRECT ANSWER-incomplete fixation Microscopic evalution of H&E stained sections from a surgically removed small bowl specimen shows an absence of much of the epithelium in otherwise normal tissue. This most likely resulted from: CORRECT...

Histotechnology CLO 5Cry techniques the use of cold temperature to fix tissues and preserve microtome a device used to make very thin slices of tissue for histology Tissue embedding process surrounding the tissue with paraffin wax to create a tissue block Glutaraldehyde and formaldehyde types of aldehydes used in fixation an example of physical fixation using heat or cold temperature microwave fixation Tissues is heated right through the block in a very short tim...

Histotechnology: A Self-Instructional Text, 4th ed. For adequate fixation, the fixative volume should be at least ____ times greater than the tissue volume. A. Equal B. 5-10 C. 10-14 D. 15-20 - correct answers 15-20 This type of microscope is used for the identification of crystals as well as to make identification of amyloid A. Light microscope B. Polarizing microscope C. Phase-contrast microscope D. Darkfield microscope - correct answers Polarizing microscope For most routine H ...

Histotechnology Lesson 5.1 and 5.2 Fixation (histology) the process of fixing tissues with chemical of physical fixatives to preserve tissues Fixative actions (5) •Preserves the tissues in a life-like condition •Stops abnormal enzyme activity and metabolism •Stops autolysis by inactivating the lysosomal enzymes •Stops bacterial and fungal growth •Stops putrefactive changes Give 2 qualities of a perfect fixative •Works quickly with minimum distortion, penetrate the t...

Histotechnology Mid Term When is H&E QC done? - correct answers -Daily -Before any patient slides are stained What is H&E QC? - correct answers -Slides are checked manually for approval -Documented and logged for future inspections What is the meniscus? - correct answers -Bottom line of the curved surface of the liquid How many grams are needed to make 500mL of the 10% solution? - correct answers % desired:100 = g needed:volume desired -50g What causes tissue to shrink, dry, br...

Histotechnology Mid TermWhen is H&E QC done? - correct answers -Daily -Before any patient slides are stained What is H&E QC? - correct answers -Slides are checked manually for approval -Documented and logged for future inspections What is the meniscus? - correct answers -Bottom line of the curved surface of the liquid How many grams are needed to make 500mL of the 10% solution? - correct answers % desired:100 = g needed:volume desired -50g What causes tissue to shrink, dry, brit...