Southern blotting - Study guides, Class notes & Summaries

QBM Exam 3 Questions And Answers With Complete Solutions 2024 Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? - answerminiprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? - answerCesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? - answerMiniprep. Which of the following wavelengths is incorrectly matched? - answer240 nm - RNA concentration. The ...

what is the purpose of Polymerase chain reaction (PCR)? correct answer: diagnostic tool to amplify a desired DNA fragment. What do you need for PCR? correct answer: Excess PRIMERS, Excess dNTPs, DNA polymerase (taq) What is the purpose of DNA polymerase ? correct answer: makes polymers of any molecules what is a chain reaction ? correct answer: a reaction that progress in an exponential way 2--4--6--8--10 why making copied of DNA ? correct answer: diagnosing infections, crime ...

BIO 1232 - SEMI FINAL PART A AND B QUESTIONS AND ANSWERS 100% CORRECT. Cell Biology 1. Which of the following is the most important type of force used to stabilize secondary structure in proteins? a) Ionic bonds b) Hydrogen bonds c) Aromatic-aromatic ring interactions d) Hydrophobic Interactions e) Peptide bonds 2. Which of the following is NOT true of allosterically regulated enzymes? a) they follow Michaelis-Menten kinetics b) they demonstrate cooperativity c) their activity can b...

Chemistry 1- Semester 1 Exam--> Chapter 4 Vocab QUESTION AND ANSWER 2024 GRADED A+ UPGRADE 2. What feature is commonly seen in the sequences recognized by type II restriction enzymes? - CORRECT ANSWERS-Solution: The recognition sequences are palindromic, and 4−8 base pairs long. 3. What normal role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from the action of restriction enzymes? - CORRECT ANSWERS-Solution: Restriction enzymes cut foreign DNA, such ...

What are the 3 types of gel electrophoresis Agarose Polyacrylamide Pulse Field What is gel electrophoresis the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis due to DNA being negatively charged, it will migrate towards the positive end What determines how far the DNA will travel in a gel the size of the DNA fragment What factors affec...

BIO 1232 - SEMI FINAL PART A AND B QUESTIONS AND ANSWERS 100% CORRECT. Cell Biology 1. Which of the following is the most important type of force used to stabilize secondary structure in proteins? a) Ionic bonds b) Hydrogen bonds c) Aromatic-aromatic ring interactions d) Hydrophobic Interactions e) Peptide bonds 2. Which of the following is NOT true of allosterically regulated enzymes? a) they follow Michaelis-Menten kinetics b) they demonstrate cooperativity c) their activity can b...

AAMC full length 2, EXAM Overviews 2023 Update amide linking is peptide on the carboxyl side - ANS-so amide linkage involving the N atom in AMC used to covalently attach flurophore to the peptide what expression gives the amount of light energy (J/photon) that is converted to other forms between the fluorescence excitation and emission events? - ANS-E=hf = hc/lambda, here we see that excitation is at 360 nm but observation is at 440 nm. so you have E = (6.62 x 10^-34)(3x 10^8)x (1/360...

AP Bio Genetics, RNA, and DNA Practice Exam 110 Questions with Verified Answers In his transformation experiments, what did Griffith observe? a. Mutant mice were resistant to bacterial infections. b. Mixing a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain can convert some of the living cells into the pathogenic form. c. Mixing a heat-killed nonpathogenic strain of bacteria with a living pathogenic strain makes the pathogenic strain nonpathogenic. d. Infecti...

Microbiology Exam 2 practice Tests 208 Questions with Verified Answers Which of the following statements is correct? A) For every amino acid, there is one codon. B) A codon may signify more than one amino acid. C) For every codon, there is one amino acid. D) All of these are correct. - CORRECT ANSWER For every Codon, there is one amino acid The sigma factor of RNA polymerase is necessary for A) maximum transcription of the gene. B) termination of the mRNA chain. C) elongation...

©THEBRIGHT EXAM STUDY SOLUTIONS 8/21/2024 8:36 PM MCB3020 Exam 3 Questions With Correct Answers Reverse transcriptase process - answer1. poly-T primer to single-strand RNA 2. reverse transcriptase to produce DNA-RNA hybrid 3. enzyme RNaseH to cut RNA and regenerate RNA primers 4, DNA polymerase and ligase synthesizes new complementary DNA strand cloning vector - answerallows for transfer of gene of interest to a host organism; allows for the making of recombinant DNA good cloning vecto...