Sugar iron agar - Study guides, Class notes & Summaries

m   1. Which of the following is the mRNA start codon in most cases? A. UAA B. AGU C. AUG D. UGA 2. Which of the types of RNA is the smallest? A. mRNA B. tRNA C. rRNA D. 3. Which of the following is not considered a pyrimidine? A. C B. T C. U D. G 4. Which of the following is paired correctly? A. A-G B. C-G C. A-U D. G-T 5. Which of the following characterizes a Western blot? A. Antibody/protein hybridization B. DNA/RNA combination C. RNA ...

TSI stands for correct answer: Triple Sugar Iron If peptone is metabolized then what is made correct answer: Ammonia What are the 3 carbohydrates present for TSI correct answer: Glucose Sucrose Lactose Glucose is a correct answer: monosaccharide Sucrose and Lactose are correct answer: disaccharides Monosaccharides differ from disaccharides by correct answer: using less energy to be broken down Ph indicator that is used is correct answer: Phenol Red The color of the ag...

Triple Sugar Iron Test Exam 38 Questions with Verified Answers For what bacteria would you use the TSI test? - CORRECT ANSWER Gram negative bacilli such as enterobacteriaceae. How would you identify an acidic slant? - CORRECT ANSWER If the slant goes from red to yellow on the phenol red. Why is there more lactose and sucrose in TSI agar than glucose? - CORRECT ANSWER In order to distinguish bacteria that can only ferment glucose from bacteria that ferment lactose and sucrose. It will al...

Microbiology Exam 2 Practice Test 47 Questions with Verified Answers Salts and sugars work to preserve foods by creating a a. hypertonic environment b. hypotonic environment c. lower pH d. lower osmotic pressure e. depletion of nutrients - CORRECT ANSWER a. hypertonic environment The term facultative anaerobe refers to an organism that a. uses oxygen or grows without oxygen b. requires less oxygen than is present in air c. is killed by oxygen d. doesn't use oxygen but tolerat...

Microbiology Lab: KIA/TSI Test 31 Questions with Verified Answers What five things are being tested in a KIA/TSI test? - CORRECT ANSWER 1. Glucose fermentation 2. Lactose fermentation 3. Sulfur reduction 4. Gas production 5. Amino acid degeneration What happens to the color of the tube when glucose is fermented? - CORRECT ANSWER The sugar is fermented and acid drops the pH, this causes the tube to turn yellow When the butt of the slant is yellow but the rest of the slant is pink...

What is the role of resazurin in fluid thioglycolate medium... - Answer-Resazurin indicates the presence of oxygen in the media A bacterial isolate is subcultured to two blood agar plates, one of which is incubated in an anaerobe jar, and the other is incubated on a shelf in the incubator. After incubation, growth is observed on the plate from the anaerobe jar, but there is not growth on the plate incubated on the incubator shelf. Based on these results how would this organism be categorize...

Types of Tests (4) - ANSWER-TSI Citrate Carbohydrate Fermentation Test w/ Durham Tubes MR/VP TSI Test - ANSWER-Triple Sugar Iron Test (slant) both a streak and a slant Citrate test - ANSWER-typically only a streak is done but both a streak and slant may be performed MR/VP test - ANSWER-Methyl Red Voges- Proskauer TSI Composition - ANSWER-Basic T Soy Agar plus: 1. glucose, lactose, & sucrose. Lac and Suc are 10x the conc. of glucose 2. Phenol red (pH indicator) pH 6...

What does TSI stand for? correct answer: Triple Sugar Iron How do you inoculate a TSI? correct answer: Stab and Drag What does a TSI slant agar select for? correct answer: Differentiates between non-fermenters, glucose fermenters and fermenters of lactose and/or sucrose. It is used to identify intestinal bacteria. What does a black medium on SIM agar indicate? correct answer: Positive for sulfur reduction and H₂S production What agent do you add to the indole test and what orga...

TSI (Triple Sugar Iron) Test 32 Questions with Verified Answers List the tests that are involved with TSI. - CORRECT ANSWER H2S Gas and Carbohydrate Fermentation What is the media for H2S Gas? - CORRECT ANSWER TSI agar What method do you use to place content on the media? - CORRECT ANSWER stab and drag motion what is the substrate for H2S gas? - CORRECT ANSWER cystein What is the product for H2S gas? - CORRECT ANSWER H2S What is the reagent for H2S gas? - CORRECT ANSWER iron in...