Bcmb 406b lab 1 exam - Study guides, Class notes & Summaries

The success of a PCR reaction is mostly dependent on what? - well designed primers Primer design can also be applied to what? - - probes in Southern and Northern blots - DNA sequencing - microarray design What else can affect the success of a PCR reaction? - - Quality of the template DNA - cycling conditions - Specificity and stability of the PCR primers What are key criteria in primer design? - - Primer length - Percent GC content - Melting temperature - Sequence How are Primer...

PCR allows for: amplification of minute amounts of template DNA can generate thousands or millions of copies of that sequence What is one of the most important parameters for a successful PCR? well designed primers Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More Name 3 variables that influence the success of a PCR reaction 1. quality of template DNA 2. cycling conditions 3. specificity and stabilit...

BCMB 406B - Lab 1 Exam Questions With Correct Answers The success of a PCR reaction is mostly dependent on what? - answerwell designed primers Primer design can also be applied to what? - answer- probes in Southern and Northern blots - DNA sequencing - microarray design What else can affect the success of a PCR reaction? - answer- Quality of the template DNA - cycling conditions - Specificity and stability of the PCR primers What are key criteria in primer design? - answer- Primer length...