Bcmb 406b lab 2 - Study guides, Class notes & Summaries

BCMB 406B - Lab 2 Test Study Guide ...

Glycoside hydrolases Answer> Hydrolyze the glycosidic bond between 2 or more carbohydrates or carbohydrate-protein bonds Carbohydrate Binding Modules Answer> - Amino acid sequence within a carbohydrate-active enzyme with a discreet fold that has carbohydrate binding activity - Maintains the interaction of the enzyme with its substrate Vector NTI Advance Answer> - Allows you to plan your experiment and ensure that it results in the desired product Benefits of in silico Answ...

Purpose of SDS-NaOH and Sodium Acetate in Alkaline lysis method? - Answer SDS- NaOH: achieves cell lysis - Chromosomal DNA remains in a high molecular weight form but is fully denatured Sodium Acetate Solution: neutralizes solution - Chromosomal DNA randomly base pairs and becomes insoluble - High salt concentration precipitates SDS-protein complexes and high molecular weight DNA

Glycoside hydrolases - Hydrolyze the glycosidic bond between 2 or more carbohydrates or carbohydrate-protein bonds Carbohydrate Binding Modules - - Amino acid sequence within a carbohydrate-active enzyme with a discreet fold that has carbohydrate binding activity - Maintains the interaction of the enzyme with its substrate Vector NTI Advance - - Allows you to plan your experiment and ensure that it results in the desired product Benefits of in silico - - Predicts restriction fragment s...

0.1 ng vs. 1 ng pET28a w/ Kan selection - Answer - Showed that the amount of pET28a plasmid DNA doesn't affect the transformation efficiency 0.1 ng vs 0.1 ng (1/10 dilution) pUC19 w/ Kan selection - Answer - Showed that the amount of pUC19 present influenced the transformation efficiency Untreated CBM26-3 PCR product - Answer - Shows that the WT DNA can be transformed and grow on a plate without the DpnI treatment

Glycoside hydrolases Hydrolyze the glycosidic bond between 2 or more carbohydrates or carbohydrate-protein bonds Carbohydrate Binding Modules - Amino acid sequence within a carbohydrate-active enzyme with a discreet fold that has carbohydrate binding activity - Maintains the interaction of the enzyme with its substrate Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More Vector NTI Advance - Allows you to...