Cuvette - Study guides, Class notes & Summaries

Beer's law says... Correct Answer-Absorbance is proportional to concentration Beer's law formula and purpose Correct Answer-A = ϵlc A: absorbance (measured by the spectrophotometer) ϵ: extinction coefficient l: path length of light through sample (usually 1 cm - printed on the cuvette) c: concentration To calculate concentration Absorbance formula Correct Answer-A = -log(I/Io) I: intensity of incident light Io: intensity of transmitted light To measure absorbance Beer...

Diurnal variation -Answer Changes in concentration of an analyte based on the time of the day (measured at day vs. night time) What is increased in the AM? (b/c of diurnal variation) -Answer ACTH, Cortisol, Iron ACTH -Answer adrenocorticotropic hormone- stimulates adrenal cortex to secrete CORTISOL How does stress affect chemistry values? -Answer Increase in ACTH, cortisol, and catecholamines Catecholamines -Answer Hormones made by adrenal glands (on top of kidneys) du...

the importance of TMS (CH3)4Si - correct answers -TMS is extremely shielded (more so than most organics) -1 sharp spike at 0, due to 12 equivalent protons -chem. Inert -low BP, easily removed Beer's Law - correct answers A=abc A=absorbance a=molar absorptivity b=path length c=concentration know how to find c= if λ=max then a is constant if a standardized cuvette is used b is constant if A, b, and a are known, then c can be calculated, but only if all those criteria are met! (A ...

MLS Exam 4 Questions & Answers 2024/2025 absorbance spectrophotometry - ANSWERSthe concentration of an unknown sample is determined by measuring its absorption of light at a particular wavelength and comparing it with the absorption of light by known standard solutions measured at the same time and with the same wavelength. absorbed - ANSWERSall other wavelengths that are not seen by the eye Beer's Law - ANSWERSthe amount of light absorbed by a solution is directly proportional to th...

Heme lab practical 2 MLT to MLS UAMS Exam Questions With Verified Answers. Pros about automated counting - answerSpeed Accuracy Eliminates subjectivity Precision More cells are counted Small blood volume Pros about Manuel counting - answerLow Cost Good for low work volume Fewer artifacts and spurious results Impedance - answerDetects and measures changes in electrical resistance between two electrodes as cells pass through a sensing aperture How is the cell population evaluated on a...

MMSC407 Final Exam Questions with Correct Answers Describe the characteristics of light as an energy source. - Answer-1. Wavelength - the distance between the crests of two successive waves 2. Amplitude - the height of each wave 3. Frequency - the number of waves which pass an observation point in one second In terms of energy: -High energy = high frequency, short wavelength -Low energy = low frequency, long wavelength Describe the relationship between percent transmittance and abso...

Closely packed purple nucleated cells" - ️️thymus A class is studying bgalactosidase activity in a bacterial culture. After a student adds ONPG to their sample, the student adds two drops of chloroform, mixes the samples, and allows the samples to sit before transferring the supernatant to a cuvette for a enzymatic assay reading using a spectrophotometry. To the student's surprise, their enzymatic assay reading was significantly lower than the rest of the class's. What is the best explan...

______________ cells are striated, tubular, and multi-nucleated. They are classified as voluntary and their structure allows for locomotion and movement. - ️️Skeletal muscle _______________ is the rapid spread of disease to a large population over a short period of time, and ______________ is when _____________ strikes at a global scale - ️️Epidemic, pandemic, epidemic 1 microgram (μg) is equal to how many milligrams (mg)? - ️️0.001 5' AGGTCTAATCCGTGTGAGCGGCGAC-[(AAAGTGTAGCCAGTA...

MMSC 407 Final Exam questions and answers well illustrated. light - correct energy source that can be characterized in terms of wavelength, amplitude and frequency -the visible spectrum wavelength - correct distance between waves amplitude - correct height of a wave frequency - correct many waves pass in one second (related to speed) high energy waves - correct frequency, very short and compact wavelengths -e.g. violet low energy waves - correct frequency, lon...

__ cells are striated, tubular, and multi-nucleated. They are classified as voluntary and their structure allows for locomotion and movement. - ️️skeletal muscle 1 microgram (μg) is equal to how many milligrams (mg)? - ️️0.001 A class is studying b-galactosidase activity in a bacterial culture. After a student adds ONPG to their sample and incubates for 10 minutes, the student then adds 10 uL PopCulture, mixes the samples, and allows the samples to sit briefly before taking an enzymat...