MB (ASCP) Exam Questions and Answers Graded A+
Predominant form of DNA Right-handed B
DNA and histone interaction is due to what type of bond? Ionic
What stains to the minor groove of DNA duplex? SYBR Green
What is the most abundant type of RNA? Ribosomal RNA (rRNA)
What does RNA act as in...
MB (ASCP) Exam Questions and Answers Graded A+
Predominant form of DNA ✔✔ Right-handed B
DNA and histone interaction is due to what type of bond? ✔✔ Ionic
What stains to the minor groove of DNA duplex? ✔✔ SYBR Green
What is the most abundant type of RNA? ✔✔ Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? ✔✔ Genome and mRNA
What increases half-life of mRNA? ✔✔ 5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? ✔✔ Euchromatin
What is the most common type of eukaryotic DNA? ✔✔ Satellite DNA
What specimen has the most DNA according to molecular weight? ✔✔ White Blood
Cells
What amino acids compose histones? ✔✔ Lysine and Arginine
Amino acid that all proteins start with: ✔✔ Methionine
What protects the DNA strand during replication? ✔✔ Single-stranded binding protein
Leading Strand ✔✔ 3' to 5' template
Lagging Strand ✔✔ 5' to 3' template
What DNA strand is being copied in transcription? ✔✔ Antisense Strand
First step after newly formed mRNA is made? ✔✔ Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme?
✔✔ Telomerase
What polymerase has repair activity? ✔✔ Beta/Epsilon
What polymerase acts on leading strand? ✔✔ Delta
What polymerase acts on lagging strand? ✔✔ Alpha
, Which polymerase is used for mitochondrial replication and repair? ✔✔ Gamma
What polymerase binds the 5' end to 3' end of dsDNA? ✔✔ Topoisomerase
Which RNA polymerase is mainly for transcription? ✔✔ RNA Pol II
Which polymerase is involved with mRNA synthesis? ✔✔ RNA Pol II
What inactivates RNAses? ✔✔ Diethyl pyrocarbonate (DEPC) or bake glassware for 4
to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? ✔✔ Restriction Enzyme
Type II
What type of restriction enzyme lacks methylation activity? ✔✔ Restriction Enzyme
Type II
What is necessary to make cDNA? ✔✔ Reverse transcriptase
Number of SNPs in a human genome? ✔✔ 11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? ✔✔ 3 billion base pairs
The polymerization of acrylamide requires: ✔✔ Catalyst (APS or TEMED) or Light
activation
How does agarose gel pore size relate to concentration? ✔✔ Inversely proportional
What is denatured during a southern blot? ✔✔ Hydrogen bonds (inter-strand bonds)
Melting temperature formula ✔✔ Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? ✔✔ 5' G
Proper GC content of primer ✔✔ 35-65%
Inhibitors of PCR ✔✔ Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol
Blue
Order to add to a PCR reaction: ✔✔ Master mix > patient sample > positive control >
negative control
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